Uji Kepekaan Sel Biofilm Isolat Klinik Pseudomonas aeruginosa Penyebab OMSK di RSUP dr. Sardjito Terhadap Gentamicin
Background : P.aeruginosa is one of many bacteria that can cause Supppurative Chronic Otitis Media. It can form a biofilm which is difficult to treat because it can defend itself against various antibiotic. Objective : The objective of this research was to do a susceptibility test to a biofilm forme...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| author | , ADI BAGUS ANDRIANTO , dr. Agus Surono, Sp.THT.KL, Ph.D |
| author_facet | , ADI BAGUS ANDRIANTO , dr. Agus Surono, Sp.THT.KL, Ph.D |
| author_sort | , ADI BAGUS ANDRIANTO |
| collection | UGM |
| description | Background : P.aeruginosa is one of many bacteria that can cause
Supppurative Chronic Otitis Media. It can form a biofilm which
is difficult to treat because it can defend itself against
various antibiotic.
Objective : The objective of this research was to do a
susceptibility test to a biofilm formed by P.aeruginosa on
Chronic Suppurative Otitis Media patients against gentamicin in
vitro.
Method and Material : Design of this research is observational
analitic, which is cross sectional. Biofilm susceptibility test
against gentamicin done by microbroth dilution method using 96-
well microplate. Concentration of gentamicin was 128, 64, 32,
16, 8, 4, 2, 1, 0.5, and 0.25 g/ml. After putting antibiotic,
MTT assay is performed. Results of MTT assay is analysed with
MBRC50.
Results : There is no concentration founded to have absorbance
below MBRC50 cut-off point. Gentamicin concentration that come
close to MBRC50 cut-off is at 128 g/ml, which can reduce biofilm
cell quantity of P.aeruginosa to the point of 48.5%.
Conclusion : Pseudomonas aeruginosa biofilm on CSOM patients who
came to THT clinic RSUP dr.Sardjito, given the gentamicin in
vitro founded to have highest MBRC of 48.5% at concentration of
128 g/ml. |
| first_indexed | 2024-03-13T23:20:16Z |
| format | Thesis |
| id | oai:generic.eprints.org:128230 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:20:16Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1282302016-03-04T07:51:32Z https://repository.ugm.ac.id/128230/ Uji Kepekaan Sel Biofilm Isolat Klinik Pseudomonas aeruginosa Penyebab OMSK di RSUP dr. Sardjito Terhadap Gentamicin , ADI BAGUS ANDRIANTO , dr. Agus Surono, Sp.THT.KL, Ph.D ETD Background : P.aeruginosa is one of many bacteria that can cause Supppurative Chronic Otitis Media. It can form a biofilm which is difficult to treat because it can defend itself against various antibiotic. Objective : The objective of this research was to do a susceptibility test to a biofilm formed by P.aeruginosa on Chronic Suppurative Otitis Media patients against gentamicin in vitro. Method and Material : Design of this research is observational analitic, which is cross sectional. Biofilm susceptibility test against gentamicin done by microbroth dilution method using 96- well microplate. Concentration of gentamicin was 128, 64, 32, 16, 8, 4, 2, 1, 0.5, and 0.25 g/ml. After putting antibiotic, MTT assay is performed. Results of MTT assay is analysed with MBRC50. Results : There is no concentration founded to have absorbance below MBRC50 cut-off point. Gentamicin concentration that come close to MBRC50 cut-off is at 128 g/ml, which can reduce biofilm cell quantity of P.aeruginosa to the point of 48.5%. Conclusion : Pseudomonas aeruginosa biofilm on CSOM patients who came to THT clinic RSUP dr.Sardjito, given the gentamicin in vitro founded to have highest MBRC of 48.5% at concentration of 128 g/ml. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , ADI BAGUS ANDRIANTO and , dr. Agus Surono, Sp.THT.KL, Ph.D (2014) Uji Kepekaan Sel Biofilm Isolat Klinik Pseudomonas aeruginosa Penyebab OMSK di RSUP dr. Sardjito Terhadap Gentamicin. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68568 |
| spellingShingle | ETD , ADI BAGUS ANDRIANTO , dr. Agus Surono, Sp.THT.KL, Ph.D Uji Kepekaan Sel Biofilm Isolat Klinik Pseudomonas aeruginosa Penyebab OMSK di RSUP dr. Sardjito Terhadap Gentamicin |
| title | Uji Kepekaan Sel Biofilm Isolat Klinik Pseudomonas aeruginosa Penyebab OMSK di RSUP dr. Sardjito Terhadap
Gentamicin |
| title_full | Uji Kepekaan Sel Biofilm Isolat Klinik Pseudomonas aeruginosa Penyebab OMSK di RSUP dr. Sardjito Terhadap
Gentamicin |
| title_fullStr | Uji Kepekaan Sel Biofilm Isolat Klinik Pseudomonas aeruginosa Penyebab OMSK di RSUP dr. Sardjito Terhadap
Gentamicin |
| title_full_unstemmed | Uji Kepekaan Sel Biofilm Isolat Klinik Pseudomonas aeruginosa Penyebab OMSK di RSUP dr. Sardjito Terhadap
Gentamicin |
| title_short | Uji Kepekaan Sel Biofilm Isolat Klinik Pseudomonas aeruginosa Penyebab OMSK di RSUP dr. Sardjito Terhadap
Gentamicin |
| title_sort | uji kepekaan sel biofilm isolat klinik pseudomonas aeruginosa penyebab omsk di rsup dr sardjito terhadap gentamicin |
| topic | ETD |
| work_keys_str_mv | AT adibagusandrianto ujikepekaanselbiofilmisolatklinikpseudomonasaeruginosapenyebabomskdirsupdrsardjitoterhadapgentamicin AT dragussuronospthtklphd ujikepekaanselbiofilmisolatklinikpseudomonasaeruginosapenyebabomskdirsupdrsardjitoterhadapgentamicin |