Aktivitas Antiplasmodium, Penghambatan Polimerisasi Heme, dan Sitotoksisitas Ekstrak Air Daun Benalu (Dendrophthoe pentandra) secara in vitro
Background: Malaria is one of the most prevalent tropical disease in the world. Until now, the use of antimalarials generated a lot of resistance that many scientists were developing research using herbal plant ingredients, for example mistletoe (D. pentandra). Previous studies have shown that D. pe...
| Main Authors: | , |
|---|---|
| Format: | Thesis |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2014
|
| Subjects: |
| _version_ | 1826047989181841408 |
|---|---|
| author | , DANANG SETIA BUDI , Dr. dr. Eti Nurwening Sholikhah, M.Kes |
| author_facet | , DANANG SETIA BUDI , Dr. dr. Eti Nurwening Sholikhah, M.Kes |
| author_sort | , DANANG SETIA BUDI |
| collection | UGM |
| description | Background: Malaria is one of the most prevalent
tropical disease in the world. Until now, the use of
antimalarials generated a lot of resistance that many
scientists were developing research using herbal plant
ingredients, for example mistletoe (D. pentandra).
Previous studies have shown that D. pentandra have
compound that has antimalarial activity.
Objective: To determine in vitro antiplasmodial
activity, mechanism of heme polymerization inhibition,
and cytotoxicity of water extract of D. pentandra.
Method: Antiplasmodium test conducted on P. falciparum
using Giemsa staining. The heme polymerization
inhibition test was conducted using hematin with test
compounds using glacial acetic acid 100% and NaOH 0.1M.
Cytotoxicity test performed on Vero cells that had been
incubated with test compound.
Result: The water extract of D. pentandra has
antiplasmodial activity with IC50 values of 45,39μg/mL.
The antiplasmodium mechanism of water extract occurs
through inhibition of heme polymerization with IC50
values of 5.49μg/mL. The cytotoxicity IC50 values is
1394.4μg/mL. Selectivity index of water extract of D.
pentandra leaves is 30,72.
Conclusion: Dendrophthoe pentandra has in vitro
antiplasmodial activity against P. falciparum through
the mechanism of heme polymerization inhibition with
low cytotoxicity. |
| first_indexed | 2024-03-13T23:20:19Z |
| format | Thesis |
| id | oai:generic.eprints.org:128249 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:20:19Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1282492016-03-04T07:54:07Z https://repository.ugm.ac.id/128249/ Aktivitas Antiplasmodium, Penghambatan Polimerisasi Heme, dan Sitotoksisitas Ekstrak Air Daun Benalu (Dendrophthoe pentandra) secara in vitro , DANANG SETIA BUDI , Dr. dr. Eti Nurwening Sholikhah, M.Kes ETD Background: Malaria is one of the most prevalent tropical disease in the world. Until now, the use of antimalarials generated a lot of resistance that many scientists were developing research using herbal plant ingredients, for example mistletoe (D. pentandra). Previous studies have shown that D. pentandra have compound that has antimalarial activity. Objective: To determine in vitro antiplasmodial activity, mechanism of heme polymerization inhibition, and cytotoxicity of water extract of D. pentandra. Method: Antiplasmodium test conducted on P. falciparum using Giemsa staining. The heme polymerization inhibition test was conducted using hematin with test compounds using glacial acetic acid 100% and NaOH 0.1M. Cytotoxicity test performed on Vero cells that had been incubated with test compound. Result: The water extract of D. pentandra has antiplasmodial activity with IC50 values of 45,39μg/mL. The antiplasmodium mechanism of water extract occurs through inhibition of heme polymerization with IC50 values of 5.49μg/mL. The cytotoxicity IC50 values is 1394.4μg/mL. Selectivity index of water extract of D. pentandra leaves is 30,72. Conclusion: Dendrophthoe pentandra has in vitro antiplasmodial activity against P. falciparum through the mechanism of heme polymerization inhibition with low cytotoxicity. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , DANANG SETIA BUDI and , Dr. dr. Eti Nurwening Sholikhah, M.Kes (2014) Aktivitas Antiplasmodium, Penghambatan Polimerisasi Heme, dan Sitotoksisitas Ekstrak Air Daun Benalu (Dendrophthoe pentandra) secara in vitro. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68587 |
| spellingShingle | ETD , DANANG SETIA BUDI , Dr. dr. Eti Nurwening Sholikhah, M.Kes Aktivitas Antiplasmodium, Penghambatan Polimerisasi Heme, dan Sitotoksisitas Ekstrak Air Daun Benalu (Dendrophthoe pentandra) secara in vitro |
| title | Aktivitas Antiplasmodium, Penghambatan Polimerisasi Heme, dan Sitotoksisitas
Ekstrak Air Daun Benalu (Dendrophthoe pentandra) secara in vitro |
| title_full | Aktivitas Antiplasmodium, Penghambatan Polimerisasi Heme, dan Sitotoksisitas
Ekstrak Air Daun Benalu (Dendrophthoe pentandra) secara in vitro |
| title_fullStr | Aktivitas Antiplasmodium, Penghambatan Polimerisasi Heme, dan Sitotoksisitas
Ekstrak Air Daun Benalu (Dendrophthoe pentandra) secara in vitro |
| title_full_unstemmed | Aktivitas Antiplasmodium, Penghambatan Polimerisasi Heme, dan Sitotoksisitas
Ekstrak Air Daun Benalu (Dendrophthoe pentandra) secara in vitro |
| title_short | Aktivitas Antiplasmodium, Penghambatan Polimerisasi Heme, dan Sitotoksisitas
Ekstrak Air Daun Benalu (Dendrophthoe pentandra) secara in vitro |
| title_sort | aktivitas antiplasmodium penghambatan polimerisasi heme dan sitotoksisitas ekstrak air daun benalu dendrophthoe pentandra secara in vitro |
| topic | ETD |
| work_keys_str_mv | AT danangsetiabudi aktivitasantiplasmodiumpenghambatanpolimerisasihemedansitotoksisitasekstrakairdaunbenaludendrophthoepentandrasecarainvitro AT drdretinurweningsholikhahmkes aktivitasantiplasmodiumpenghambatanpolimerisasihemedansitotoksisitasekstrakairdaunbenaludendrophthoepentandrasecarainvitro |