PENGARUH FREKUENSI SUBKULTUR KALUS TEBU (Saccharum officinarum L.) TERHADAP KEMAMPUAN BERTUNAS DAN KERAGAMAN GENETIK BIBIT BERDASARKAN PENANDA RAPD
In vitro culture technique offers unique opportunity to create the genetic variability which called somaclonal variation. The aims of this study are determine the effect of frequency of callus subculture on plant regeneration and genetic stability of sugarcane clones. The influence of frequency of c...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| _version_ | 1826048040219181056 |
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| author | , Rulita Puspitasari , Dr. Ir. Taryono, M.Sc |
| author_facet | , Rulita Puspitasari , Dr. Ir. Taryono, M.Sc |
| author_sort | , Rulita Puspitasari |
| collection | UGM |
| description | In vitro culture technique offers unique opportunity to create the genetic
variability which called somaclonal variation. The aims of this study are
determine the effect of frequency of callus subculture on plant regeneration and
genetic stability of sugarcane clones. The influence of frequency of callus
subculture for regeneration ability among sugarcane clones (BZ 121, Q 81, Q 83,
and Kentung) were evaluated, with treatment of callus subculture frequency were
0 (S0), 1 (S1), 2 (S2), 3 (S3), 4 (S4), and 5 (S5). The sliced tissue from young
leaves roll (5 mm) were used as explants. MS containing 3 mg/l 2,4-D medium
was used for callus induction, mass of callus were maintained by subculturing
every 4 weeks with MS containing 3 mg/l 2,4-D medium and regenerated in MS
containing 2 mg/l IAA + 2 mg/l IBA + 2 mg/l Kinetin medium. After 8 weeks
regenerated, sugarcane plantlets subcultured in MS containing 2 mg/l NAA
rooting medium. The regeneration of callus among sugarcane clones (BZ 121, Q
81, Q 83, and Kentung) decreased after third subculture and no shoots regenerate
on fourth callus subculture. Genetic variation in sugarcane clones were vary
depending on interaction with subculture frequency. Based on RAPD markers,
there were different increased on genetic variability among clones. The percentage
of polimorphic of BZ 121 was the lowest, whereas Q 81 was the higest. Based on
subculture frequency, genetic variability of first subculture was the lowest,
whereas direct regeneration was the higest. |
| first_indexed | 2024-03-13T23:21:11Z |
| format | Thesis |
| id | oai:generic.eprints.org:128505 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:21:11Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1285052016-03-04T08:11:30Z https://repository.ugm.ac.id/128505/ PENGARUH FREKUENSI SUBKULTUR KALUS TEBU (Saccharum officinarum L.) TERHADAP KEMAMPUAN BERTUNAS DAN KERAGAMAN GENETIK BIBIT BERDASARKAN PENANDA RAPD , Rulita Puspitasari , Dr. Ir. Taryono, M.Sc ETD In vitro culture technique offers unique opportunity to create the genetic variability which called somaclonal variation. The aims of this study are determine the effect of frequency of callus subculture on plant regeneration and genetic stability of sugarcane clones. The influence of frequency of callus subculture for regeneration ability among sugarcane clones (BZ 121, Q 81, Q 83, and Kentung) were evaluated, with treatment of callus subculture frequency were 0 (S0), 1 (S1), 2 (S2), 3 (S3), 4 (S4), and 5 (S5). The sliced tissue from young leaves roll (5 mm) were used as explants. MS containing 3 mg/l 2,4-D medium was used for callus induction, mass of callus were maintained by subculturing every 4 weeks with MS containing 3 mg/l 2,4-D medium and regenerated in MS containing 2 mg/l IAA + 2 mg/l IBA + 2 mg/l Kinetin medium. After 8 weeks regenerated, sugarcane plantlets subcultured in MS containing 2 mg/l NAA rooting medium. The regeneration of callus among sugarcane clones (BZ 121, Q 81, Q 83, and Kentung) decreased after third subculture and no shoots regenerate on fourth callus subculture. Genetic variation in sugarcane clones were vary depending on interaction with subculture frequency. Based on RAPD markers, there were different increased on genetic variability among clones. The percentage of polimorphic of BZ 121 was the lowest, whereas Q 81 was the higest. Based on subculture frequency, genetic variability of first subculture was the lowest, whereas direct regeneration was the higest. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , Rulita Puspitasari and , Dr. Ir. Taryono, M.Sc (2014) PENGARUH FREKUENSI SUBKULTUR KALUS TEBU (Saccharum officinarum L.) TERHADAP KEMAMPUAN BERTUNAS DAN KERAGAMAN GENETIK BIBIT BERDASARKAN PENANDA RAPD. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68854 |
| spellingShingle | ETD , Rulita Puspitasari , Dr. Ir. Taryono, M.Sc PENGARUH FREKUENSI SUBKULTUR KALUS TEBU (Saccharum officinarum L.) TERHADAP KEMAMPUAN BERTUNAS DAN KERAGAMAN GENETIK BIBIT BERDASARKAN PENANDA RAPD |
| title | PENGARUH FREKUENSI SUBKULTUR KALUS TEBU
(Saccharum officinarum L.) TERHADAP
KEMAMPUAN BERTUNAS DAN KERAGAMAN GENETIK BIBIT
BERDASARKAN PENANDA RAPD |
| title_full | PENGARUH FREKUENSI SUBKULTUR KALUS TEBU
(Saccharum officinarum L.) TERHADAP
KEMAMPUAN BERTUNAS DAN KERAGAMAN GENETIK BIBIT
BERDASARKAN PENANDA RAPD |
| title_fullStr | PENGARUH FREKUENSI SUBKULTUR KALUS TEBU
(Saccharum officinarum L.) TERHADAP
KEMAMPUAN BERTUNAS DAN KERAGAMAN GENETIK BIBIT
BERDASARKAN PENANDA RAPD |
| title_full_unstemmed | PENGARUH FREKUENSI SUBKULTUR KALUS TEBU
(Saccharum officinarum L.) TERHADAP
KEMAMPUAN BERTUNAS DAN KERAGAMAN GENETIK BIBIT
BERDASARKAN PENANDA RAPD |
| title_short | PENGARUH FREKUENSI SUBKULTUR KALUS TEBU
(Saccharum officinarum L.) TERHADAP
KEMAMPUAN BERTUNAS DAN KERAGAMAN GENETIK BIBIT
BERDASARKAN PENANDA RAPD |
| title_sort | pengaruh frekuensi subkultur kalus tebu saccharum officinarum l terhadap kemampuan bertunas dan keragaman genetik bibit berdasarkan penanda rapd |
| topic | ETD |
| work_keys_str_mv | AT rulitapuspitasari pengaruhfrekuensisubkulturkalustebusaccharumofficinarumlterhadapkemampuanbertunasdankeragamangenetikbibitberdasarkanpenandarapd AT drirtaryonomsc pengaruhfrekuensisubkulturkalustebusaccharumofficinarumlterhadapkemampuanbertunasdankeragamangenetikbibitberdasarkanpenandarapd |