KONVERSI MIKROBIOLOGIS BATANG POHON SINGKONG (Mannihot esculenta) MENJADI ETANOL OLEH Trichoderma reesei PK1J2 DAN Saccharomyces cerevisiae 3012
Cassava stems can be utilized as an alternative energy source to produce bioethanol due to its cellulose content, polysaccharide compound can not directly used by yeast. Therefore, cellulose need to be hydrolyzed into monomers prior to tranformation into ethanol by Saccharomyces cerevisiae. Hydrolys...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| Summary: | Cassava stems can be utilized as an alternative energy source to produce
bioethanol due to its cellulose content, polysaccharide compound can not directly
used by yeast. Therefore, cellulose need to be hydrolyzed into monomers prior to
tranformation into ethanol by Saccharomyces cerevisiae. Hydrolysis can be
performed by cellulase-producing fungi, such as Trichoderma reesei PK1J2 with
ammonium sulphate used as nitrogen source. This study purposes were to analyze
the effect of ammonium sulphate addition to the growth and reducing sugar
production rate of Trichoderma reesei from cassava stems and also to analyze the
effect of fermentation method on Saccharomyces cerevisiae ethanol production.
Hydrolysis was conducted without pretreatment. Cassava stem was
degraded into small pieces with and without addition of ammonium sulphate. The
concentration of (NH4)2SO4 were 0% |
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