POTENSI AKTIVITAS ANTIVIRAL EKSTRAK ETIL ASETAT DAN EKSTRAK AIR METABOLIT SEKUNDER AKTINOMISETES TERHADAP VIRUS DENGUE SEROTIPE-2 (DENV-2)
Dengue virus (DENV) infections are one of the most prevalent infectious diseases in many parts of the world and affecting millions of people annualy. Despite the growing threat from the spread of DENV, there is still no approved antiviral drug for DENV infections. Actinomycetes are well known produc...
| Main Authors: | , |
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| _version_ | 1797035033233457152 |
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| author | , Dimas Fandi Praditya , dr. Tri Wibawa, Ph.D. |
| author_facet | , Dimas Fandi Praditya , dr. Tri Wibawa, Ph.D. |
| author_sort | , Dimas Fandi Praditya |
| collection | UGM |
| description | Dengue virus (DENV) infections are one of the most prevalent infectious
diseases in many parts of the world and affecting millions of people annualy. Despite
the growing threat from the spread of DENV, there is still no approved antiviral drug
for DENV infections. Actinomycetes are well known producers of pharmacological
and antiviral agents. Here, we screening ethyl acetate and aqueous extracts from
actinomycetes (GMY01, GMY16, GMY29, GMY57, GMR06 and GMR22) for their
antiviral activity against dengue virus serotype 2 (DENV-2). BHK-21 cell were
infected with DENV-2 and treated with ethyl acetate and aqueous extract. The
antiviral activity were analyzed by semi-quantitative RT-PCR and evaluated base on
the inhibition of DENV-2 replication. Results revealed all extract from all isolates
inhibits in vitro DENV-2 replication in dose dependent maner. The highest inhibition
activity was found at the concentration 25 μg/ml for all extract ranging from 80-90 %.
These results show the potency of aqueous and ethyl acetate extract from marine
actinomycetes as DENV-2 antiviral candidate. |
| first_indexed | 2024-03-13T23:22:21Z |
| format | Thesis |
| id | oai:generic.eprints.org:128711 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:22:21Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1287112016-03-04T07:55:18Z https://repository.ugm.ac.id/128711/ POTENSI AKTIVITAS ANTIVIRAL EKSTRAK ETIL ASETAT DAN EKSTRAK AIR METABOLIT SEKUNDER AKTINOMISETES TERHADAP VIRUS DENGUE SEROTIPE-2 (DENV-2) , Dimas Fandi Praditya , dr. Tri Wibawa, Ph.D. ETD Dengue virus (DENV) infections are one of the most prevalent infectious diseases in many parts of the world and affecting millions of people annualy. Despite the growing threat from the spread of DENV, there is still no approved antiviral drug for DENV infections. Actinomycetes are well known producers of pharmacological and antiviral agents. Here, we screening ethyl acetate and aqueous extracts from actinomycetes (GMY01, GMY16, GMY29, GMY57, GMR06 and GMR22) for their antiviral activity against dengue virus serotype 2 (DENV-2). BHK-21 cell were infected with DENV-2 and treated with ethyl acetate and aqueous extract. The antiviral activity were analyzed by semi-quantitative RT-PCR and evaluated base on the inhibition of DENV-2 replication. Results revealed all extract from all isolates inhibits in vitro DENV-2 replication in dose dependent maner. The highest inhibition activity was found at the concentration 25 μg/ml for all extract ranging from 80-90 %. These results show the potency of aqueous and ethyl acetate extract from marine actinomycetes as DENV-2 antiviral candidate. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , Dimas Fandi Praditya and , dr. Tri Wibawa, Ph.D. (2014) POTENSI AKTIVITAS ANTIVIRAL EKSTRAK ETIL ASETAT DAN EKSTRAK AIR METABOLIT SEKUNDER AKTINOMISETES TERHADAP VIRUS DENGUE SEROTIPE-2 (DENV-2). UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=69077 |
| spellingShingle | ETD , Dimas Fandi Praditya , dr. Tri Wibawa, Ph.D. POTENSI AKTIVITAS ANTIVIRAL EKSTRAK ETIL ASETAT DAN EKSTRAK AIR METABOLIT SEKUNDER AKTINOMISETES TERHADAP VIRUS DENGUE SEROTIPE-2 (DENV-2) |
| title | POTENSI AKTIVITAS ANTIVIRAL EKSTRAK ETIL ASETAT DAN
EKSTRAK AIR METABOLIT SEKUNDER AKTINOMISETES TERHADAP
VIRUS DENGUE SEROTIPE-2 (DENV-2) |
| title_full | POTENSI AKTIVITAS ANTIVIRAL EKSTRAK ETIL ASETAT DAN
EKSTRAK AIR METABOLIT SEKUNDER AKTINOMISETES TERHADAP
VIRUS DENGUE SEROTIPE-2 (DENV-2) |
| title_fullStr | POTENSI AKTIVITAS ANTIVIRAL EKSTRAK ETIL ASETAT DAN
EKSTRAK AIR METABOLIT SEKUNDER AKTINOMISETES TERHADAP
VIRUS DENGUE SEROTIPE-2 (DENV-2) |
| title_full_unstemmed | POTENSI AKTIVITAS ANTIVIRAL EKSTRAK ETIL ASETAT DAN
EKSTRAK AIR METABOLIT SEKUNDER AKTINOMISETES TERHADAP
VIRUS DENGUE SEROTIPE-2 (DENV-2) |
| title_short | POTENSI AKTIVITAS ANTIVIRAL EKSTRAK ETIL ASETAT DAN
EKSTRAK AIR METABOLIT SEKUNDER AKTINOMISETES TERHADAP
VIRUS DENGUE SEROTIPE-2 (DENV-2) |
| title_sort | potensi aktivitas antiviral ekstrak etil asetat dan ekstrak air metabolit sekunder aktinomisetes terhadap virus dengue serotipe 2 denv 2 |
| topic | ETD |
| work_keys_str_mv | AT dimasfandipraditya potensiaktivitasantiviralekstraketilasetatdanekstrakairmetabolitsekunderaktinomisetesterhadapvirusdengueserotipe2denv2 AT drtriwibawaphd potensiaktivitasantiviralekstraketilasetatdanekstrakairmetabolitsekunderaktinomisetesterhadapvirusdengueserotipe2denv2 |