KARAKTERISASI MOLEKULAR COAT PROTEIN GENE PAPAYA RINGSPOT VIRUS PADA TANAMAN PEPAYA (Carica papaya L.) DI INDONESIA
Papaya ringspot virus (PRSV), the member of Potyvirus, is commonly occurred on papaya plants (Carica papaya L.) and caused a serious damage and significant loss in papaya crops troughout the world. These symptoms are mosaic and mottle on leaves, stunted growth, leaf and fruit deformation, and also d...
| Main Authors: | , |
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| Format: | Thesis |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| _version_ | 1797035040666812416 |
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| author | , UTARI SARASWATI , Dr. Budi Setiadi Daryono, M.Agr.Sc. |
| author_facet | , UTARI SARASWATI , Dr. Budi Setiadi Daryono, M.Agr.Sc. |
| author_sort | , UTARI SARASWATI |
| collection | UGM |
| description | Papaya ringspot virus (PRSV), the member of Potyvirus, is commonly
occurred on papaya plants (Carica papaya L.) and caused a serious damage and
significant loss in papaya crops troughout the world. These symptoms are mosaic
and mottle on leaves, stunted growth, leaf and fruit deformation, and also dark
green ringspots on fruits. To date, there is no report about the occurrences of
PRSV infecting papaya in Indonesia, but surveys on several papaya fields in The
Province of Yogyakarta and Central Java Indonesia during 2011 � 2013 showed
the similar symptoms that caused by PRSV. The objective of this research were to
find out the occurrences of PRSV infecting papaya in Indonesia and to
characterize its coat protein (CP) gene on molecular level. Survey and collecting
samples randomly conducted in several papaya fields in Sleman, Bantul,
Gunungkidul and Kulonprogo Yogyakarta, and also in Kebumen, Boyolali and
Magelang Central Java. Result of serological test by DAS-ELISA indicated that
18 symptomic papaya leaf samples was positive PRSV-infected. Virus particles
observation using TEM showed flexuous filament structure of PRSV. Host range
analysis with mechanical inoculation showed common symptoms, such as mosaic,
necrosis and leaf distortion on Carica papaya and Cucumis melo, and also
necrotic local lesion on Chenopodium amaranticolor 6 � 8 weeks after
inoculation. Detection of the CP gene of PRSV by RT-PCR revealed + 449 bp in
length of DNA fragment on agarose gel electrophoresis. Molecular
characterization of partial sequences of the CP gene showed percent identities of
49,51 � 53,92% for PRSV-Kebumen1 and 51,26 � 56,78% for PRSV-Kebumen2.
Based on phylogeny tree, its phylogenetic relationship are far enough from other
PRSV isolates. Hence, we concluded that the new Indonesia PRSV isolates have
evolved by a series of nucleotides mutation. |
| first_indexed | 2024-03-13T23:22:28Z |
| format | Thesis |
| id | oai:generic.eprints.org:128748 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:22:28Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1287482016-03-04T08:16:14Z https://repository.ugm.ac.id/128748/ KARAKTERISASI MOLEKULAR COAT PROTEIN GENE PAPAYA RINGSPOT VIRUS PADA TANAMAN PEPAYA (Carica papaya L.) DI INDONESIA , UTARI SARASWATI , Dr. Budi Setiadi Daryono, M.Agr.Sc. ETD Papaya ringspot virus (PRSV), the member of Potyvirus, is commonly occurred on papaya plants (Carica papaya L.) and caused a serious damage and significant loss in papaya crops troughout the world. These symptoms are mosaic and mottle on leaves, stunted growth, leaf and fruit deformation, and also dark green ringspots on fruits. To date, there is no report about the occurrences of PRSV infecting papaya in Indonesia, but surveys on several papaya fields in The Province of Yogyakarta and Central Java Indonesia during 2011 � 2013 showed the similar symptoms that caused by PRSV. The objective of this research were to find out the occurrences of PRSV infecting papaya in Indonesia and to characterize its coat protein (CP) gene on molecular level. Survey and collecting samples randomly conducted in several papaya fields in Sleman, Bantul, Gunungkidul and Kulonprogo Yogyakarta, and also in Kebumen, Boyolali and Magelang Central Java. Result of serological test by DAS-ELISA indicated that 18 symptomic papaya leaf samples was positive PRSV-infected. Virus particles observation using TEM showed flexuous filament structure of PRSV. Host range analysis with mechanical inoculation showed common symptoms, such as mosaic, necrosis and leaf distortion on Carica papaya and Cucumis melo, and also necrotic local lesion on Chenopodium amaranticolor 6 � 8 weeks after inoculation. Detection of the CP gene of PRSV by RT-PCR revealed + 449 bp in length of DNA fragment on agarose gel electrophoresis. Molecular characterization of partial sequences of the CP gene showed percent identities of 49,51 � 53,92% for PRSV-Kebumen1 and 51,26 � 56,78% for PRSV-Kebumen2. Based on phylogeny tree, its phylogenetic relationship are far enough from other PRSV isolates. Hence, we concluded that the new Indonesia PRSV isolates have evolved by a series of nucleotides mutation. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , UTARI SARASWATI and , Dr. Budi Setiadi Daryono, M.Agr.Sc. (2014) KARAKTERISASI MOLEKULAR COAT PROTEIN GENE PAPAYA RINGSPOT VIRUS PADA TANAMAN PEPAYA (Carica papaya L.) DI INDONESIA. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=69114 |
| spellingShingle | ETD , UTARI SARASWATI , Dr. Budi Setiadi Daryono, M.Agr.Sc. KARAKTERISASI MOLEKULAR COAT PROTEIN GENE PAPAYA RINGSPOT VIRUS PADA TANAMAN PEPAYA (Carica papaya L.) DI INDONESIA |
| title | KARAKTERISASI MOLEKULAR COAT PROTEIN GENE PAPAYA RINGSPOT VIRUS PADA TANAMAN PEPAYA
(Carica papaya L.) DI INDONESIA |
| title_full | KARAKTERISASI MOLEKULAR COAT PROTEIN GENE PAPAYA RINGSPOT VIRUS PADA TANAMAN PEPAYA
(Carica papaya L.) DI INDONESIA |
| title_fullStr | KARAKTERISASI MOLEKULAR COAT PROTEIN GENE PAPAYA RINGSPOT VIRUS PADA TANAMAN PEPAYA
(Carica papaya L.) DI INDONESIA |
| title_full_unstemmed | KARAKTERISASI MOLEKULAR COAT PROTEIN GENE PAPAYA RINGSPOT VIRUS PADA TANAMAN PEPAYA
(Carica papaya L.) DI INDONESIA |
| title_short | KARAKTERISASI MOLEKULAR COAT PROTEIN GENE PAPAYA RINGSPOT VIRUS PADA TANAMAN PEPAYA
(Carica papaya L.) DI INDONESIA |
| title_sort | karakterisasi molekular coat protein gene papaya ringspot virus pada tanaman pepaya carica papaya l di indonesia |
| topic | ETD |
| work_keys_str_mv | AT utarisaraswati karakterisasimolekularcoatproteingenepapayaringspotviruspadatanamanpepayacaricapapayaldiindonesia AT drbudisetiadidaryonomagrsc karakterisasimolekularcoatproteingenepapayaringspotviruspadatanamanpepayacaricapapayaldiindonesia |