PURIFIKASI DAN KARAKTERISASI MERKURI REDUKTASE Isolat Streptomyces spp. RIZOSFER RUMPUT TEKI DI DAERAH TERCEMAR MERKURI
Purification and characterization mercuric reductase four isolates Streptomyces (Streptomyces sp. AS1, Streptomyces sp. AS2, Streptomyces sp. AS6, and Streptomyces sp. BR28) have been investigated. Cell free extract was obtained by disrupting cells using sea sand at 4 °C and followed by centrifugat...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| _version_ | 1797035046070124544 |
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| author | , HANUM MUKTI RAHAYU , Dr. Yekti Asih Purwestri, S.Si, M.Si |
| author_facet | , HANUM MUKTI RAHAYU , Dr. Yekti Asih Purwestri, S.Si, M.Si |
| author_sort | , HANUM MUKTI RAHAYU |
| collection | UGM |
| description | Purification and characterization mercuric reductase four isolates Streptomyces
(Streptomyces sp. AS1, Streptomyces sp. AS2, Streptomyces sp. AS6, and
Streptomyces sp. BR28) have been investigated. Cell free extract was obtained by
disrupting cells using sea sand at 4 °C and followed by centrifugation. Mercuric
reductase was purified by ammonium sulfat precipitation, dialysis, and
chromatography column (DEAE Sepharose anion column chromatography).
Determination pH and temperature optimum of mercuric reductase activity based
on the number of NADPH2 oxidized to NADP per mg protein per minute by using
a spectrophotometer. Molecular weight mercuric reductase determined using
SDS-PAGE. The result showed spesific activity of mercuric reductase
Streptomyces sp. BR28 was the highest. The optimum pH and temperature cellfree
extract enzyme mercuric reductase were 7,5 and 80 °C, respectively. The
enzyme was purified to 431,87-fold with spesific activity 21918,95 U/mg protein.
SDS PAGE showed that Streptomyces sp. BR 28 has mercuric reductase. It can be
concluded that Streptomyces isolates have mercuric reductase and be potential as a
bioremediation agent. |
| first_indexed | 2024-03-13T23:22:33Z |
| format | Thesis |
| id | oai:generic.eprints.org:128775 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:22:33Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1287752016-03-04T07:56:43Z https://repository.ugm.ac.id/128775/ PURIFIKASI DAN KARAKTERISASI MERKURI REDUKTASE Isolat Streptomyces spp. RIZOSFER RUMPUT TEKI DI DAERAH TERCEMAR MERKURI , HANUM MUKTI RAHAYU , Dr. Yekti Asih Purwestri, S.Si, M.Si ETD Purification and characterization mercuric reductase four isolates Streptomyces (Streptomyces sp. AS1, Streptomyces sp. AS2, Streptomyces sp. AS6, and Streptomyces sp. BR28) have been investigated. Cell free extract was obtained by disrupting cells using sea sand at 4 °C and followed by centrifugation. Mercuric reductase was purified by ammonium sulfat precipitation, dialysis, and chromatography column (DEAE Sepharose anion column chromatography). Determination pH and temperature optimum of mercuric reductase activity based on the number of NADPH2 oxidized to NADP per mg protein per minute by using a spectrophotometer. Molecular weight mercuric reductase determined using SDS-PAGE. The result showed spesific activity of mercuric reductase Streptomyces sp. BR28 was the highest. The optimum pH and temperature cellfree extract enzyme mercuric reductase were 7,5 and 80 °C, respectively. The enzyme was purified to 431,87-fold with spesific activity 21918,95 U/mg protein. SDS PAGE showed that Streptomyces sp. BR 28 has mercuric reductase. It can be concluded that Streptomyces isolates have mercuric reductase and be potential as a bioremediation agent. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , HANUM MUKTI RAHAYU and , Dr. Yekti Asih Purwestri, S.Si, M.Si (2014) PURIFIKASI DAN KARAKTERISASI MERKURI REDUKTASE Isolat Streptomyces spp. RIZOSFER RUMPUT TEKI DI DAERAH TERCEMAR MERKURI. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=69141 |
| spellingShingle | ETD , HANUM MUKTI RAHAYU , Dr. Yekti Asih Purwestri, S.Si, M.Si PURIFIKASI DAN KARAKTERISASI MERKURI REDUKTASE Isolat Streptomyces spp. RIZOSFER RUMPUT TEKI DI DAERAH TERCEMAR MERKURI |
| title | PURIFIKASI DAN KARAKTERISASI MERKURI REDUKTASE Isolat Streptomyces spp.
RIZOSFER RUMPUT TEKI DI DAERAH TERCEMAR MERKURI |
| title_full | PURIFIKASI DAN KARAKTERISASI MERKURI REDUKTASE Isolat Streptomyces spp.
RIZOSFER RUMPUT TEKI DI DAERAH TERCEMAR MERKURI |
| title_fullStr | PURIFIKASI DAN KARAKTERISASI MERKURI REDUKTASE Isolat Streptomyces spp.
RIZOSFER RUMPUT TEKI DI DAERAH TERCEMAR MERKURI |
| title_full_unstemmed | PURIFIKASI DAN KARAKTERISASI MERKURI REDUKTASE Isolat Streptomyces spp.
RIZOSFER RUMPUT TEKI DI DAERAH TERCEMAR MERKURI |
| title_short | PURIFIKASI DAN KARAKTERISASI MERKURI REDUKTASE Isolat Streptomyces spp.
RIZOSFER RUMPUT TEKI DI DAERAH TERCEMAR MERKURI |
| title_sort | purifikasi dan karakterisasi merkuri reduktase isolat streptomyces spp rizosfer rumput teki di daerah tercemar merkuri |
| topic | ETD |
| work_keys_str_mv | AT hanummuktirahayu purifikasidankarakterisasimerkurireduktaseisolatstreptomycesspprizosferrumputtekididaerahtercemarmerkuri AT dryektiasihpurwestrissimsi purifikasidankarakterisasimerkurireduktaseisolatstreptomycesspprizosferrumputtekididaerahtercemarmerkuri |