ANALISIS FREKUENSI MIKRONUKLEUS PADA SEL EPITEL MUKOSA BUKAL PEKERJA DRY CLEANING DI YOGYAKARTA
Dry cleaning solvent which is commonly used such as perchloroethylene was detected having potential of genotoxic effects. Genotoxic effects of substances can cause DNA damage that affects in the formation of micronucleus which could serve as indicator of DNA damage in human. Evaluation of micronucle...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| Summary: | Dry cleaning solvent which is commonly used such as perchloroethylene
was detected having potential of genotoxic effects. Genotoxic effects of
substances can cause DNA damage that affects in the formation of micronucleus
which could serve as indicator of DNA damage in human. Evaluation of
micronucleus in buccal mucosal epithelial cells is a non-invasive and simple
method to examine micronucleus frequencies. The aim of this study was to
evaluate the exposure effect and duration of exposure effect of perchloroethylene
in the increase of micronucleus frequencies in buccal mucosal epithelial cells of
dry cleaning workers in Yogyakarta.
This cross sectional study involved 12 subjects and 12 controls. The subjects
were male dry cleaning workers who were exposed to perchloroethylene, while
controls were healthy male students who were not exposed to perchloroethylene.
Buccal mucosal epithelial cells were taken using cytobrush and smeared on object
glass containing droplets of NaCl 0.09% solution and then fixed in methanolacetate.
The specimens were stained using modified Feulgen-Rossenbeck method.
Micronucleus frequencies were scored per 1000 cells under light microscope. The
data then analyzed by independent t test and pearson correlation test on p<0,05
significances.
The results showed that there was significant difference in micronucleus
frequencies between group of dry cleaning workers and the control group
however duration of exposure time did not correlate with the increase of
micronucleus frequencies in the group of dry cleaning workers. This study
concluded that exposure of perchloroethylene indicates affects increase of
micronucleus frequencies in buccal mucosal epithelial cells of dry cleaning
workers in Yogyakarta. Duration of exposure time of perchloroethylene did not
correlate with the increase of micronucleus frequencies in buccal mucosal
epithelial cells of dry cleaning workers in Yogyakarta. |
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