Pigment stability studies in selected pigment-producing microbes from New Zealand environment

The industrial production of natural food colourants is already well-established and expanding. However, the range of natural colour-shades is still limited compared to synthetic dyes. Besides, the use of plant extracts is known to be expensive and uncompetitive to synthetic dyes due to their high p...

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Main Authors: Kamarudin, Kamarul Rahim, Lewis, Gillian, Greenwood, David R., Villas-Bôas, Silas
Format: Proceeding Paper
Language:English
Published: 2009
Subjects:
Online Access:http://irep.iium.edu.my/12952/1/Abstract_ComBio_2009_KAMARUL.pdf
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author Kamarudin, Kamarul Rahim
Lewis, Gillian
Greenwood, David R.
Villas-Bôas, Silas
author_facet Kamarudin, Kamarul Rahim
Lewis, Gillian
Greenwood, David R.
Villas-Bôas, Silas
author_sort Kamarudin, Kamarul Rahim
collection IIUM
description The industrial production of natural food colourants is already well-established and expanding. However, the range of natural colour-shades is still limited compared to synthetic dyes. Besides, the use of plant extracts is known to be expensive and uncompetitive to synthetic dyes due to their high production costs. Consequently, microorganisms are becoming a more popular alternative source for natural food grade pigments. Development of microbial food grade pigments are likely to cut down the high production cost of natural colours, thus leading to a cheaper source of natural food colourants among the modern consumers. Preceding commercialization, pigment stability and toxicity assessment are required in order to determine their suitability to the food industry. We, therefore, have screened 286 pigment-producing microbial strains isolated from New Zealand environment. Based on water solubility and colour-shades of interest, 41 pigments have been short-listed for pigment stability tests towards different pHs, temperatures and light. To date, our current results showed that 81% of the yellow-to-reddish water-soluble pigments and the red SVB-B50 water-insoluble pigment were stable at all ranges of temperature i.e. -20oC, 4oC, 60oC, 100oC and microwave heat. Light and pH stability tests are being carried out. Further to this study, metabolomics tools will be incorporated to elucidate the metabolic pathway for biosynthesis of a selected microbial pigment with most potential for industrial application, and metabolic engineering strategies will be determined aiming for improvement of the pigment production yields during fermentation.
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spelling oai:generic.eprints.org:129522012-01-27T02:41:08Z http://irep.iium.edu.my/12952/ Pigment stability studies in selected pigment-producing microbes from New Zealand environment Kamarudin, Kamarul Rahim Lewis, Gillian Greenwood, David R. Villas-Bôas, Silas Q Science (General) The industrial production of natural food colourants is already well-established and expanding. However, the range of natural colour-shades is still limited compared to synthetic dyes. Besides, the use of plant extracts is known to be expensive and uncompetitive to synthetic dyes due to their high production costs. Consequently, microorganisms are becoming a more popular alternative source for natural food grade pigments. Development of microbial food grade pigments are likely to cut down the high production cost of natural colours, thus leading to a cheaper source of natural food colourants among the modern consumers. Preceding commercialization, pigment stability and toxicity assessment are required in order to determine their suitability to the food industry. We, therefore, have screened 286 pigment-producing microbial strains isolated from New Zealand environment. Based on water solubility and colour-shades of interest, 41 pigments have been short-listed for pigment stability tests towards different pHs, temperatures and light. To date, our current results showed that 81% of the yellow-to-reddish water-soluble pigments and the red SVB-B50 water-insoluble pigment were stable at all ranges of temperature i.e. -20oC, 4oC, 60oC, 100oC and microwave heat. Light and pH stability tests are being carried out. Further to this study, metabolomics tools will be incorporated to elucidate the metabolic pathway for biosynthesis of a selected microbial pigment with most potential for industrial application, and metabolic engineering strategies will be determined aiming for improvement of the pigment production yields during fermentation. 2009-12-06 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/12952/1/Abstract_ComBio_2009_KAMARUL.pdf Kamarudin, Kamarul Rahim and Lewis, Gillian and Greenwood, David R. and Villas-Bôas, Silas (2009) Pigment stability studies in selected pigment-producing microbes from New Zealand environment. In: ComBio 2009, 6-10 December 2009, Christchurch Convention Centre, Christchurch, New Zealand. http://www.canterbury.ac.nz/conference/combio09/docs/Abstracts.pdf
spellingShingle Q Science (General)
Kamarudin, Kamarul Rahim
Lewis, Gillian
Greenwood, David R.
Villas-Bôas, Silas
Pigment stability studies in selected pigment-producing microbes from New Zealand environment
title Pigment stability studies in selected pigment-producing microbes from New Zealand environment
title_full Pigment stability studies in selected pigment-producing microbes from New Zealand environment
title_fullStr Pigment stability studies in selected pigment-producing microbes from New Zealand environment
title_full_unstemmed Pigment stability studies in selected pigment-producing microbes from New Zealand environment
title_short Pigment stability studies in selected pigment-producing microbes from New Zealand environment
title_sort pigment stability studies in selected pigment producing microbes from new zealand environment
topic Q Science (General)
url http://irep.iium.edu.my/12952/1/Abstract_ComBio_2009_KAMARUL.pdf
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AT greenwooddavidr pigmentstabilitystudiesinselectedpigmentproducingmicrobesfromnewzealandenvironment
AT villasboassilas pigmentstabilitystudiesinselectedpigmentproducingmicrobesfromnewzealandenvironment