Antioxidant study of methanolic and ethanolic extracts of Malaysian aromatic plants’ leaves: towards industrial biotechnology
The aim of this study is to determine the antioxidant properties of methanolic and ethanolic extracts of four Malaysian aromatic plants’ leaves, namely knotweed (Polygonum minus), curry (Murraya koenigii), kaffir lime (Citrus hysrix) and fragrant screwpine (Pandanus odurus) as a head start for indus...
| Main Authors: | , , , , , |
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| Format: | Proceeding Paper |
| Language: | English |
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2010
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| Online Access: | http://irep.iium.edu.my/12960/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf |
| _version_ | 1796876019001458688 |
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| author | Kamarudin, Kamarul Rahim Abu Abas, Azlim Almey Koya, Mustapha Suleiman Syed Osman Idid, Syed Zahir Idid Mohamed Rehan, Aisyah Khan Chowdhury, Ahmed Jalal |
| author_facet | Kamarudin, Kamarul Rahim Abu Abas, Azlim Almey Koya, Mustapha Suleiman Syed Osman Idid, Syed Zahir Idid Mohamed Rehan, Aisyah Khan Chowdhury, Ahmed Jalal |
| author_sort | Kamarudin, Kamarul Rahim |
| collection | IIUM |
| description | The aim of this study is to determine the antioxidant properties of methanolic and ethanolic extracts of four Malaysian aromatic plants’ leaves, namely knotweed (Polygonum minus), curry (Murraya koenigii), kaffir lime (Citrus hysrix) and fragrant screwpine (Pandanus odurus) as a head start for industrial biotechnology. Total phenolic content (TPC) assay using Folin-Ciocalteu method was used to assess the presence and level of phenolic compounds in each sample. Antioxidant activity in terms of total antioxidant and free radical scavenging activities of both methanolic and ethanolic extracts was measured by β-carotene bleaching assay and 2, 2, diphenyl-1- icrylhydrazyl (DPPH) free radical scavenging activity assay, respectively. The present results showed that methanolic extract of P. minus exhibited the highest total antioxidant activity (98.2 ±0.5%) while for the EC50 based on DPPH. radical scavenging activity assay, the lowest concentration was shown by P. minus extracts as compared to the other samples. For both ethanolic and methanolic extracts, moderately strong and strong positive correlations existed between the phenolic content and antioxidant activity based on β-carotene bleaching assay, respectively. Among the eluent systems used for phytochemical analysis using thin layer chromatography (TLC), EWFA showed the largest number of antioxidant active compounds, followed by EMW and CEF. HEA eluent system indicated no presence of antioxidant active compound showing that most compounds were polar. Retardation factor (Rf) values were calculated between 0.2 to 0.6. Relatively, the present results suggested that of the four aromatic plants, P. minus and M. koenigii have high potential as sources of commercial natural antioxidants regardless types of extracting solvents. |
| first_indexed | 2024-03-05T22:46:34Z |
| format | Proceeding Paper |
| id | oai:generic.eprints.org:12960 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:46:34Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:129602017-12-06T04:54:13Z http://irep.iium.edu.my/12960/ Antioxidant study of methanolic and ethanolic extracts of Malaysian aromatic plants’ leaves: towards industrial biotechnology Kamarudin, Kamarul Rahim Abu Abas, Azlim Almey Koya, Mustapha Suleiman Syed Osman Idid, Syed Zahir Idid Mohamed Rehan, Aisyah Khan Chowdhury, Ahmed Jalal Q Science (General) The aim of this study is to determine the antioxidant properties of methanolic and ethanolic extracts of four Malaysian aromatic plants’ leaves, namely knotweed (Polygonum minus), curry (Murraya koenigii), kaffir lime (Citrus hysrix) and fragrant screwpine (Pandanus odurus) as a head start for industrial biotechnology. Total phenolic content (TPC) assay using Folin-Ciocalteu method was used to assess the presence and level of phenolic compounds in each sample. Antioxidant activity in terms of total antioxidant and free radical scavenging activities of both methanolic and ethanolic extracts was measured by β-carotene bleaching assay and 2, 2, diphenyl-1- icrylhydrazyl (DPPH) free radical scavenging activity assay, respectively. The present results showed that methanolic extract of P. minus exhibited the highest total antioxidant activity (98.2 ±0.5%) while for the EC50 based on DPPH. radical scavenging activity assay, the lowest concentration was shown by P. minus extracts as compared to the other samples. For both ethanolic and methanolic extracts, moderately strong and strong positive correlations existed between the phenolic content and antioxidant activity based on β-carotene bleaching assay, respectively. Among the eluent systems used for phytochemical analysis using thin layer chromatography (TLC), EWFA showed the largest number of antioxidant active compounds, followed by EMW and CEF. HEA eluent system indicated no presence of antioxidant active compound showing that most compounds were polar. Retardation factor (Rf) values were calculated between 0.2 to 0.6. Relatively, the present results suggested that of the four aromatic plants, P. minus and M. koenigii have high potential as sources of commercial natural antioxidants regardless types of extracting solvents. 2010-01-26 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/12960/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf Kamarudin, Kamarul Rahim and Abu Abas, Azlim Almey and Koya, Mustapha Suleiman and Syed Osman Idid, Syed Zahir Idid and Mohamed Rehan, Aisyah and Khan Chowdhury, Ahmed Jalal (2010) Antioxidant study of methanolic and ethanolic extracts of Malaysian aromatic plants’ leaves: towards industrial biotechnology. In: IIUM Research, Innovation & Invention Exhibition (IRIIE 2010), 26 - 27 January 2010, Kuala Lumpur. (Unpublished) |
| spellingShingle | Q Science (General) Kamarudin, Kamarul Rahim Abu Abas, Azlim Almey Koya, Mustapha Suleiman Syed Osman Idid, Syed Zahir Idid Mohamed Rehan, Aisyah Khan Chowdhury, Ahmed Jalal Antioxidant study of methanolic and ethanolic extracts of Malaysian aromatic plants’ leaves: towards industrial biotechnology |
| title | Antioxidant study of methanolic and ethanolic extracts of Malaysian aromatic plants’ leaves: towards industrial biotechnology |
| title_full | Antioxidant study of methanolic and ethanolic extracts of Malaysian aromatic plants’ leaves: towards industrial biotechnology |
| title_fullStr | Antioxidant study of methanolic and ethanolic extracts of Malaysian aromatic plants’ leaves: towards industrial biotechnology |
| title_full_unstemmed | Antioxidant study of methanolic and ethanolic extracts of Malaysian aromatic plants’ leaves: towards industrial biotechnology |
| title_short | Antioxidant study of methanolic and ethanolic extracts of Malaysian aromatic plants’ leaves: towards industrial biotechnology |
| title_sort | antioxidant study of methanolic and ethanolic extracts of malaysian aromatic plants leaves towards industrial biotechnology |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/12960/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf |
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