| Summary: | We have incorporated a morphological approach using ossicles of sea cucumber
(Echinodermata: Holothuroidea) and partial sequences of 16S ribosomal RNA (rRNA)
gene and cytochrome c oxidase I (COI) gene in this study as addition to partial
cytochrome b (cyt b) mitochondrial DNA (mtDNA) gene sequences for species
identification. Beside Holothuria species including Holothuria leucospilota, species from
other genera were also included in order to get a better view on the phylogenetic
relationship of Holothuria species. 27 partial sequences of 16S rRNA mtDNA gene
(Accession No.: FJ223854 � FJ223872) and COI mtDNA gene (Accession No.:
FJ223873 - FJ223880) have been registered with GenBank, National Center for
Biotechnology Information (NCBI), U.S. National Library of Medicine
(http://www.ncbi.nlm.nih.gov/) prior to publications. Sea Cucumber Database, Kulliyyah
of Science, International Islamic University Malaysia
( http://sea.cucumber.kos.iium.googlepages.com/) containing all results of this study �
the first online database on sea cucumber in Malaysia � has been developed and the
database can also be accessed worldwide from Malaysia Maritime Database
(http://marin-dbase.com/).
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