INTERAKSI GEN PADA POLA PEWARISAN JENGGER AYAM (Gallus gallus domesticus Linn., 1758) HASIL PERSILANGAN AYAM KAMPUNG DENGAN BACKCROSS II

Comb is an accessory organ on the head of chicken which is influenced by testosterone hormone and can be used as indicator of chicken�s fertility. Therefore this research was aimed to study the gene interaction of chicken comb inheritance from crossbreed between local chicken which has a pea comb...

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Main Authors: , TANJUNG ARDO, , Dr. Budi Setiadi Daryono, M.Agr.Sc
Format: Thesis
Published: [Yogyakarta] : Universitas Gadjah Mada 2014
Subjects:
ETD
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author , TANJUNG ARDO
, Dr. Budi Setiadi Daryono, M.Agr.Sc
author_facet , TANJUNG ARDO
, Dr. Budi Setiadi Daryono, M.Agr.Sc
author_sort , TANJUNG ARDO
collection UGM
description Comb is an accessory organ on the head of chicken which is influenced by testosterone hormone and can be used as indicator of chicken�s fertility. Therefore this research was aimed to study the gene interaction of chicken comb inheritance from crossbreed between local chicken which has a pea comb and the backcross II which has a single comb and also to observe SOX5 gene polymorphism. The methods used in this research were taking care of parents and day old chick (DOC), blood collection, DNA isolation, Polymerase Chain Reaction (PCR), and Restriction Fragment Length Polymorphism (RFLP) using HindIII restriction enzyme. The results showed that crossbreed between local chicken and the backcross II have 2 different offspring. This crossbreed between pea comb (rrP-) and single comb (rrpp) showed the offspring have 100% pea comb. This result cannot to be determined the genotype of female parent with pea comb is rrPP or rrPp because lack of the amount of offspring. While genotype of DOC is heterozygote pea (rrPp). The result of SOX5 gene polymorphism showed that there was no polymorphism between pea and single comb. Conclusions of this research were the qualitative phenotype of offspring were different. Genotype of female parent which has pea comb cannot to be determined and there was no polymorphism of SOX5 between pea and single comb.
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spelling oai:generic.eprints.org:1299572016-03-04T08:14:43Z https://repository.ugm.ac.id/129957/ INTERAKSI GEN PADA POLA PEWARISAN JENGGER AYAM (Gallus gallus domesticus Linn., 1758) HASIL PERSILANGAN AYAM KAMPUNG DENGAN BACKCROSS II , TANJUNG ARDO , Dr. Budi Setiadi Daryono, M.Agr.Sc ETD Comb is an accessory organ on the head of chicken which is influenced by testosterone hormone and can be used as indicator of chicken�s fertility. Therefore this research was aimed to study the gene interaction of chicken comb inheritance from crossbreed between local chicken which has a pea comb and the backcross II which has a single comb and also to observe SOX5 gene polymorphism. The methods used in this research were taking care of parents and day old chick (DOC), blood collection, DNA isolation, Polymerase Chain Reaction (PCR), and Restriction Fragment Length Polymorphism (RFLP) using HindIII restriction enzyme. The results showed that crossbreed between local chicken and the backcross II have 2 different offspring. This crossbreed between pea comb (rrP-) and single comb (rrpp) showed the offspring have 100% pea comb. This result cannot to be determined the genotype of female parent with pea comb is rrPP or rrPp because lack of the amount of offspring. While genotype of DOC is heterozygote pea (rrPp). The result of SOX5 gene polymorphism showed that there was no polymorphism between pea and single comb. Conclusions of this research were the qualitative phenotype of offspring were different. Genotype of female parent which has pea comb cannot to be determined and there was no polymorphism of SOX5 between pea and single comb. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , TANJUNG ARDO and , Dr. Budi Setiadi Daryono, M.Agr.Sc (2014) INTERAKSI GEN PADA POLA PEWARISAN JENGGER AYAM (Gallus gallus domesticus Linn., 1758) HASIL PERSILANGAN AYAM KAMPUNG DENGAN BACKCROSS II. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=70366
spellingShingle ETD
, TANJUNG ARDO
, Dr. Budi Setiadi Daryono, M.Agr.Sc
INTERAKSI GEN PADA POLA PEWARISAN JENGGER AYAM (Gallus gallus domesticus Linn., 1758) HASIL PERSILANGAN AYAM KAMPUNG DENGAN BACKCROSS II
title INTERAKSI GEN PADA POLA PEWARISAN JENGGER AYAM (Gallus gallus domesticus Linn., 1758) HASIL PERSILANGAN AYAM KAMPUNG DENGAN BACKCROSS II
title_full INTERAKSI GEN PADA POLA PEWARISAN JENGGER AYAM (Gallus gallus domesticus Linn., 1758) HASIL PERSILANGAN AYAM KAMPUNG DENGAN BACKCROSS II
title_fullStr INTERAKSI GEN PADA POLA PEWARISAN JENGGER AYAM (Gallus gallus domesticus Linn., 1758) HASIL PERSILANGAN AYAM KAMPUNG DENGAN BACKCROSS II
title_full_unstemmed INTERAKSI GEN PADA POLA PEWARISAN JENGGER AYAM (Gallus gallus domesticus Linn., 1758) HASIL PERSILANGAN AYAM KAMPUNG DENGAN BACKCROSS II
title_short INTERAKSI GEN PADA POLA PEWARISAN JENGGER AYAM (Gallus gallus domesticus Linn., 1758) HASIL PERSILANGAN AYAM KAMPUNG DENGAN BACKCROSS II
title_sort interaksi gen pada pola pewarisan jengger ayam gallus gallus domesticus linn 1758 hasil persilangan ayam kampung dengan backcross ii
topic ETD
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AT drbudisetiadidaryonomagrsc interaksigenpadapolapewarisanjenggerayamgallusgallusdomesticuslinn1758hasilpersilanganayamkampungdenganbackcrossii