| Summary: | One of the important goals of endodontic treatment is comprehensive
obturation of root canals. The success of obturation was directly related to the
adequate removal of microorganism and their by-product through mechanical root
canal instrumentation, antibacterial irrigation, adequate filling of the root canal
space and coronal restoration. Antibacterial activity is one of the desirable
properties of an ideal sealer. The purpose of this study was to evaluate the
antibacterial activity of the new resin-based root canal sealer (RealSeal) and
Calsium hydroxide-based root canal sealer (Sealapex).
Agar diffusion method was carried out in four plates previously inoculated
with Enterococcus faecalis. As base layer 10ml Mueller-Hinton agar was added
on each 20mmx100mm petri dishes. Three 6mm diameter and 2mm depth wells
were made on each well equally distance using stainless steel cylinder. 0,7ml
freshly mixed sealers were placed in prepared wells of Mueller-Hinton agar
plates. On each petri dishes, resin-based rootcanal sealer was added in the first
well as Group I, Calsium hydroxide-based rootcanal sealer was placed in the
second well as Group II and the third well remaind empty as negative control. All
plates were incubated for 24 hours at 37ºC under anaerobic condition. The
diameters of bacterial inhibition zone were measured. Statistical analysis with
homogeneity of variance test, Shapiro-Wilk test and Independent sample t-test
with the trust level 95% (α = 0,05) was applied to the data.
The average of the antibacterial activity to Enterocococcus faecalis on
group 1 was higher than on group 2. The independent sample t-test proved that
there were significant differences between the antibacterial activity of resin-based
and Calsium hydroxide-based root canal sealers against Enterococcus faecalis.
The conclusion showed that all of the sealers caused bacterial growth
inhibition. RealSeal shows superior antibacterial result over Sealapex against
Enterococcus faecalis.
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