| Summary: | Background: Solar radiation onto human skin, mainly ultraviolet A (UVA), can stimulate premature aging skin because of its effects on human dermal fibroblasts (proliferations, collagen depositions, migrations, and TGF-β consumptions). A number of evidence demonstrate that Various biological materials such as platelet lysates (PL), Wharton�s Jelly Stem Cells Conditioned Media (WJ) and Adipose Derived Stem Cells Conditioned Media (AD), may improve healing process in chronic ulcers
Objectives: This study was performed to evaluate potency of various concentration of PL, WJ, and AD in improving skin aging of human dermal fibroblasts.
Methods: A quasi experimental design was done in artificially aged human dermal fibroblasts. Fibroblast cultivated more than 3 passages (11-13 years olds boy�s foreskins were divided into two groups: Group I consisted of 10 kJ/cm2 UVA induced aged fibroblasts and Group II were normal fibroblasts without UVA irradiation as positive control. Group I were divided into 4 subgroups: subgroup 1�3 were treated by various concentration of PL, WJ, AD consecutively and subgroup 4 was a negative control. They were cultured in DMEM standard media. After treatments, they were evaluated its proliferations, migrations, collagen depositions, and level of extracellular TGF-β. Fibroblast proliferation, migration, deposition was measured by using MTT assay, Scratch assay, Insoluble Sirius Red collagen assay, respectively, while the level of TGF- was tested by using ELISA.
Results: Cultivation of aged fibroblasts in 50% PL showed significant (P<0.05) elevation of proliferations, migrations, and collagen depositions compared to negative control, but they did not have reduction of extracellulary TGF-β levels significantly (p>0.05). Cultivation of aged fibroblasts in 25% PL showed elevation of extracellulary TGF-β levels significantly (p<0.05). Proliferations, migrations, and collagen depositions of aging fibroblast culture in 50% PL were: 0.87±0.049 OD
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