LISAT PLATELET DAN MEDIUM TERKONDISI SEL PUNCA MESENSIMAL UNTUK MEMPERBAIKI KULIT MENUA DINI Kajian In-vitro pada Fibroblas Kulit Manusia Menua Dini Buatan
Background: Solar radiation onto human skin, mainly ultraviolet A (UVA), can stimulate premature aging skin because of its effects on human dermal fibroblasts (proliferations, collagen depositions, migrations, and TGF-β consumptions). A number of evidence demonstrate that Various biological materia...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| author | , Yohanes Widodo, dr., Sp.KK(K) , Prof. Dr. dr. Hardyanto Soebono, Sp.KK(K). |
| author_facet | , Yohanes Widodo, dr., Sp.KK(K) , Prof. Dr. dr. Hardyanto Soebono, Sp.KK(K). |
| author_sort | , Yohanes Widodo, dr., Sp.KK(K) |
| collection | UGM |
| description | Background: Solar radiation onto human skin, mainly ultraviolet A (UVA), can stimulate premature aging skin because of its effects on human dermal fibroblasts (proliferations, collagen depositions, migrations, and TGF-β consumptions). A number of evidence demonstrate that Various biological materials such as platelet lysates (PL), Wharton�s Jelly Stem Cells Conditioned Media (WJ) and Adipose Derived Stem Cells Conditioned Media (AD), may improve healing process in chronic ulcers
Objectives: This study was performed to evaluate potency of various concentration of PL, WJ, and AD in improving skin aging of human dermal fibroblasts.
Methods: A quasi experimental design was done in artificially aged human dermal fibroblasts. Fibroblast cultivated more than 3 passages (11-13 years olds boy�s foreskins were divided into two groups: Group I consisted of 10 kJ/cm2 UVA induced aged fibroblasts and Group II were normal fibroblasts without UVA irradiation as positive control. Group I were divided into 4 subgroups: subgroup 1�3 were treated by various concentration of PL, WJ, AD consecutively and subgroup 4 was a negative control. They were cultured in DMEM standard media. After treatments, they were evaluated its proliferations, migrations, collagen depositions, and level of extracellular TGF-β. Fibroblast proliferation, migration, deposition was measured by using MTT assay, Scratch assay, Insoluble Sirius Red collagen assay, respectively, while the level of TGF- was tested by using ELISA.
Results: Cultivation of aged fibroblasts in 50% PL showed significant (P<0.05) elevation of proliferations, migrations, and collagen depositions compared to negative control, but they did not have reduction of extracellulary TGF-β levels significantly (p>0.05). Cultivation of aged fibroblasts in 25% PL showed elevation of extracellulary TGF-β levels significantly (p<0.05). Proliferations, migrations, and collagen depositions of aging fibroblast culture in 50% PL were: 0.87±0.049 OD |
| first_indexed | 2024-03-13T23:28:52Z |
| format | Thesis |
| id | oai:generic.eprints.org:130364 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:28:52Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1303642016-03-04T08:19:00Z https://repository.ugm.ac.id/130364/ LISAT PLATELET DAN MEDIUM TERKONDISI SEL PUNCA MESENSIMAL UNTUK MEMPERBAIKI KULIT MENUA DINI Kajian In-vitro pada Fibroblas Kulit Manusia Menua Dini Buatan , Yohanes Widodo, dr., Sp.KK(K) , Prof. Dr. dr. Hardyanto Soebono, Sp.KK(K). ETD Background: Solar radiation onto human skin, mainly ultraviolet A (UVA), can stimulate premature aging skin because of its effects on human dermal fibroblasts (proliferations, collagen depositions, migrations, and TGF-β consumptions). A number of evidence demonstrate that Various biological materials such as platelet lysates (PL), Wharton�s Jelly Stem Cells Conditioned Media (WJ) and Adipose Derived Stem Cells Conditioned Media (AD), may improve healing process in chronic ulcers Objectives: This study was performed to evaluate potency of various concentration of PL, WJ, and AD in improving skin aging of human dermal fibroblasts. Methods: A quasi experimental design was done in artificially aged human dermal fibroblasts. Fibroblast cultivated more than 3 passages (11-13 years olds boy�s foreskins were divided into two groups: Group I consisted of 10 kJ/cm2 UVA induced aged fibroblasts and Group II were normal fibroblasts without UVA irradiation as positive control. Group I were divided into 4 subgroups: subgroup 1�3 were treated by various concentration of PL, WJ, AD consecutively and subgroup 4 was a negative control. They were cultured in DMEM standard media. After treatments, they were evaluated its proliferations, migrations, collagen depositions, and level of extracellular TGF-β. Fibroblast proliferation, migration, deposition was measured by using MTT assay, Scratch assay, Insoluble Sirius Red collagen assay, respectively, while the level of TGF- was tested by using ELISA. Results: Cultivation of aged fibroblasts in 50% PL showed significant (P<0.05) elevation of proliferations, migrations, and collagen depositions compared to negative control, but they did not have reduction of extracellulary TGF-β levels significantly (p>0.05). Cultivation of aged fibroblasts in 25% PL showed elevation of extracellulary TGF-β levels significantly (p<0.05). Proliferations, migrations, and collagen depositions of aging fibroblast culture in 50% PL were: 0.87±0.049 OD [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , Yohanes Widodo, dr., Sp.KK(K) and , Prof. Dr. dr. Hardyanto Soebono, Sp.KK(K). (2014) LISAT PLATELET DAN MEDIUM TERKONDISI SEL PUNCA MESENSIMAL UNTUK MEMPERBAIKI KULIT MENUA DINI Kajian In-vitro pada Fibroblas Kulit Manusia Menua Dini Buatan. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=70785 |
| spellingShingle | ETD , Yohanes Widodo, dr., Sp.KK(K) , Prof. Dr. dr. Hardyanto Soebono, Sp.KK(K). LISAT PLATELET DAN MEDIUM TERKONDISI SEL PUNCA MESENSIMAL UNTUK MEMPERBAIKI KULIT MENUA DINI Kajian In-vitro pada Fibroblas Kulit Manusia Menua Dini Buatan |
| title | LISAT PLATELET DAN MEDIUM TERKONDISI SEL PUNCA MESENSIMAL UNTUK MEMPERBAIKI
KULIT MENUA DINI Kajian In-vitro pada Fibroblas Kulit Manusia Menua Dini Buatan |
| title_full | LISAT PLATELET DAN MEDIUM TERKONDISI SEL PUNCA MESENSIMAL UNTUK MEMPERBAIKI
KULIT MENUA DINI Kajian In-vitro pada Fibroblas Kulit Manusia Menua Dini Buatan |
| title_fullStr | LISAT PLATELET DAN MEDIUM TERKONDISI SEL PUNCA MESENSIMAL UNTUK MEMPERBAIKI
KULIT MENUA DINI Kajian In-vitro pada Fibroblas Kulit Manusia Menua Dini Buatan |
| title_full_unstemmed | LISAT PLATELET DAN MEDIUM TERKONDISI SEL PUNCA MESENSIMAL UNTUK MEMPERBAIKI
KULIT MENUA DINI Kajian In-vitro pada Fibroblas Kulit Manusia Menua Dini Buatan |
| title_short | LISAT PLATELET DAN MEDIUM TERKONDISI SEL PUNCA MESENSIMAL UNTUK MEMPERBAIKI
KULIT MENUA DINI Kajian In-vitro pada Fibroblas Kulit Manusia Menua Dini Buatan |
| title_sort | lisat platelet dan medium terkondisi sel punca mesensimal untuk memperbaiki kulit menua dini kajian in vitro pada fibroblas kulit manusia menua dini buatan |
| topic | ETD |
| work_keys_str_mv | AT yohaneswidododrspkkk lisatplateletdanmediumterkondisiselpuncamesensimaluntukmemperbaikikulitmenuadinikajianinvitropadafibroblaskulitmanusiamenuadinibuatan AT profdrdrhardyantosoebonospkkk lisatplateletdanmediumterkondisiselpuncamesensimaluntukmemperbaikikulitmenuadinikajianinvitropadafibroblaskulitmanusiamenuadinibuatan |