ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI METABOLIT SEKUNDER DAUN TENDANI (Goniothalamus macrophyllus Hook.f. & Thoms)
Isolation and evaluation of antibacterial activities of secondary metabolites of tendani leaves (Goniothalamus macrophyllus Hook.f. & Thoms) have been conducted. Tendani leaves were extracted with methanol and fractionated sequentially with n-hexane and ethyl acetate. T...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| author | , AGUNG RAHMADANI , Prof. Drs. Sabirin Matsjeh, Ph.D. |
| author_facet | , AGUNG RAHMADANI , Prof. Drs. Sabirin Matsjeh, Ph.D. |
| author_sort | , AGUNG RAHMADANI |
| collection | UGM |
| description | Isolation and evaluation of antibacterial activities of secondary metabolites
of tendani leaves (Goniothalamus macrophyllus Hook.f. & Thoms) have been
conducted. Tendani leaves were extracted with methanol and fractionated
sequentially with n-hexane and ethyl acetate. The ethyl acetate extracts were
analyzed using GC-MS and separated by column chromatography to afford 7
fractions. Fractions 4 were rechromatographed to give fraction 4.3. The structure
of isolated compound was identified by Mass Spectroscopy and NMR.
The GC-MS analysis shows ethyl acetate extracts of tendani leaves contain
benzyldehyde compounds, sphatulenol, methyl hexadecanoat, 1,2-dimethoxy-1-phenyl-ethane, phytol, and stigmasterol. Fraction 4.3 is colorless needle-shape
crystals and identified as 8-acetate-7-phenyl-2,6-dioxabicyclo[3.3.1]nonane-3-one
or 9-deoxygoniopypyrone acetate. Diameters of inhibition zone of methanol
extracts, n-hexane, and ethyl acetate with concentration of 5% against
Staphylococcus aureus bacteria are 7.40, 2.50 and 6.18 mm respectively, whereas
against Escherichia coli are 6.63, 2.46 and 4.07 mm respectively. Diameters of
inhibition zone of 9-deoxygoniopypyrone acetate with concentration of 0.2%
against Staphylococcus aureus and Escherichia coli are 6.27 mm and 5.25 mm
respectively |
| first_indexed | 2024-03-13T23:29:17Z |
| format | Thesis |
| id | oai:generic.eprints.org:130473 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:29:17Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1304732016-03-04T07:51:46Z https://repository.ugm.ac.id/130473/ ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI METABOLIT SEKUNDER DAUN TENDANI (Goniothalamus macrophyllus Hook.f. & Thoms) , AGUNG RAHMADANI , Prof. Drs. Sabirin Matsjeh, Ph.D. ETD Isolation and evaluation of antibacterial activities of secondary metabolites of tendani leaves (Goniothalamus macrophyllus Hook.f. & Thoms) have been conducted. Tendani leaves were extracted with methanol and fractionated sequentially with n-hexane and ethyl acetate. The ethyl acetate extracts were analyzed using GC-MS and separated by column chromatography to afford 7 fractions. Fractions 4 were rechromatographed to give fraction 4.3. The structure of isolated compound was identified by Mass Spectroscopy and NMR. The GC-MS analysis shows ethyl acetate extracts of tendani leaves contain benzyldehyde compounds, sphatulenol, methyl hexadecanoat, 1,2-dimethoxy-1-phenyl-ethane, phytol, and stigmasterol. Fraction 4.3 is colorless needle-shape crystals and identified as 8-acetate-7-phenyl-2,6-dioxabicyclo[3.3.1]nonane-3-one or 9-deoxygoniopypyrone acetate. Diameters of inhibition zone of methanol extracts, n-hexane, and ethyl acetate with concentration of 5% against Staphylococcus aureus bacteria are 7.40, 2.50 and 6.18 mm respectively, whereas against Escherichia coli are 6.63, 2.46 and 4.07 mm respectively. Diameters of inhibition zone of 9-deoxygoniopypyrone acetate with concentration of 0.2% against Staphylococcus aureus and Escherichia coli are 6.27 mm and 5.25 mm respectively [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , AGUNG RAHMADANI and , Prof. Drs. Sabirin Matsjeh, Ph.D. (2014) ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI METABOLIT SEKUNDER DAUN TENDANI (Goniothalamus macrophyllus Hook.f. & Thoms). UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=70895 |
| spellingShingle | ETD , AGUNG RAHMADANI , Prof. Drs. Sabirin Matsjeh, Ph.D. ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI METABOLIT SEKUNDER DAUN TENDANI (Goniothalamus macrophyllus Hook.f. & Thoms) |
| title | ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI METABOLIT SEKUNDER DAUN TENDANI (Goniothalamus macrophyllus Hook.f. & Thoms) |
| title_full | ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI METABOLIT SEKUNDER DAUN TENDANI (Goniothalamus macrophyllus Hook.f. & Thoms) |
| title_fullStr | ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI METABOLIT SEKUNDER DAUN TENDANI (Goniothalamus macrophyllus Hook.f. & Thoms) |
| title_full_unstemmed | ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI METABOLIT SEKUNDER DAUN TENDANI (Goniothalamus macrophyllus Hook.f. & Thoms) |
| title_short | ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI METABOLIT SEKUNDER DAUN TENDANI (Goniothalamus macrophyllus Hook.f. & Thoms) |
| title_sort | isolasi dan uji aktivitas antibakteri metabolit sekunder daun tendani goniothalamus macrophyllus hook f thoms |
| topic | ETD |
| work_keys_str_mv | AT agungrahmadani isolasidanujiaktivitasantibakterimetabolitsekunderdauntendanigoniothalamusmacrophyllushookfthoms AT profdrssabirinmatsjehphd isolasidanujiaktivitasantibakterimetabolitsekunderdauntendanigoniothalamusmacrophyllushookfthoms |