DETEKSI DAN PENENTUAN TIPE BERDASARKAN AMPLIFIKASI DAN SEKUENSING GEN PENYANDI VIRALPROTEIN-2CANINE PARVO VIRUS PADA ANJING SUSPECT CPV
Canine parvovirus is a cause of hemorrhagic enteritis and myocarditis in dogs. This virus is a pathogenic enteric disease of dogs around the world with high morbidity ( 100 % ) and mortality up to 10 %. Canine parvovirus infection occurs very quickly and easily transmi...
| Main Authors: | , |
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| author | , wahyu peni wijayasti , Dr. drh. Aris Haryanto, M.Si. |
| author_facet | , wahyu peni wijayasti , Dr. drh. Aris Haryanto, M.Si. |
| author_sort | , wahyu peni wijayasti |
| collection | UGM |
| description | Canine parvovirus is a cause of hemorrhagic enteritis and myocarditis in
dogs. This virus is a pathogenic enteric disease of dogs around the world with
high morbidity ( 100 % ) and mortality up to 10 %. Canine parvovirus infection
occurs very quickly and easily transmitted between dogs. Therefore a quick
diagnosis is necessary to control the disease and to determine the accuracy of
disease therapy. CPV diagnosis is based on a tentative diagnosis, prognosis and
clinical examination. However, it has become difficult to diagnose because the
main clinical symptoms of gastroenteritis is similar to clinical symptoms owned
by other enteric diseases. Therefore, we need a test to detect CPV accurately and
rapidly. This study is aimed to perform the detection of the types of CPV using
diagnostic methods through amplification techniques on virus VP2 protein-coding
genes in dogs suspected of CPV lined Polymerase Chain Reaction ( PCR ).
Collection of blood samples suspected of CPV as many as 13 samples were
obtained from Animal HospitalProf. Soeparwi, Laboratorium Ilmu Penyakit
Dalam FKH UGMand Klinik Hewan Satwa Kita, Yogyakarta. Extraction is done
to get the CPV viral DNA. The results of the extraction are used as a template for
amplification by PCR using primers to the target protein-coding genes VP2.
Products produced by PCR are then visualized by electrophoresis on a 1% agarose
gel UV Transilluminator. A positive result is indicated by the appearance of a
DNA fragment of 518 bp which can be interpreted as CPV. PCR products were
subsequently sequenced to determine the sequence of the nucleotide bases.
Sequencing results then undergoes alignment with protein-coding genes of Canine
Parvovirus VP2 derived from Genbank data base using the program MEGA versi
5. Of the 13 blood samples it is confirmed that 11 are positive samples with CPV.
The type of CPV is type 2a. Conventional PCR methods can be used in detecting
CPV virus in samples derived from blood specimens from patients suspected of
CPV dog. |
| first_indexed | 2024-03-13T23:29:23Z |
| format | Thesis |
| id | oai:generic.eprints.org:130507 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:29:23Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1305072016-03-04T08:19:59Z https://repository.ugm.ac.id/130507/ DETEKSI DAN PENENTUAN TIPE BERDASARKAN AMPLIFIKASI DAN SEKUENSING GEN PENYANDI VIRALPROTEIN-2CANINE PARVO VIRUS PADA ANJING SUSPECT CPV , wahyu peni wijayasti , Dr. drh. Aris Haryanto, M.Si. ETD Canine parvovirus is a cause of hemorrhagic enteritis and myocarditis in dogs. This virus is a pathogenic enteric disease of dogs around the world with high morbidity ( 100 % ) and mortality up to 10 %. Canine parvovirus infection occurs very quickly and easily transmitted between dogs. Therefore a quick diagnosis is necessary to control the disease and to determine the accuracy of disease therapy. CPV diagnosis is based on a tentative diagnosis, prognosis and clinical examination. However, it has become difficult to diagnose because the main clinical symptoms of gastroenteritis is similar to clinical symptoms owned by other enteric diseases. Therefore, we need a test to detect CPV accurately and rapidly. This study is aimed to perform the detection of the types of CPV using diagnostic methods through amplification techniques on virus VP2 protein-coding genes in dogs suspected of CPV lined Polymerase Chain Reaction ( PCR ). Collection of blood samples suspected of CPV as many as 13 samples were obtained from Animal HospitalProf. Soeparwi, Laboratorium Ilmu Penyakit Dalam FKH UGMand Klinik Hewan Satwa Kita, Yogyakarta. Extraction is done to get the CPV viral DNA. The results of the extraction are used as a template for amplification by PCR using primers to the target protein-coding genes VP2. Products produced by PCR are then visualized by electrophoresis on a 1% agarose gel UV Transilluminator. A positive result is indicated by the appearance of a DNA fragment of 518 bp which can be interpreted as CPV. PCR products were subsequently sequenced to determine the sequence of the nucleotide bases. Sequencing results then undergoes alignment with protein-coding genes of Canine Parvovirus VP2 derived from Genbank data base using the program MEGA versi 5. Of the 13 blood samples it is confirmed that 11 are positive samples with CPV. The type of CPV is type 2a. Conventional PCR methods can be used in detecting CPV virus in samples derived from blood specimens from patients suspected of CPV dog. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , wahyu peni wijayasti and , Dr. drh. Aris Haryanto, M.Si. (2014) DETEKSI DAN PENENTUAN TIPE BERDASARKAN AMPLIFIKASI DAN SEKUENSING GEN PENYANDI VIRALPROTEIN-2CANINE PARVO VIRUS PADA ANJING SUSPECT CPV. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=70930 |
| spellingShingle | ETD , wahyu peni wijayasti , Dr. drh. Aris Haryanto, M.Si. DETEKSI DAN PENENTUAN TIPE BERDASARKAN AMPLIFIKASI DAN SEKUENSING GEN PENYANDI VIRALPROTEIN-2CANINE PARVO VIRUS PADA ANJING SUSPECT CPV |
| title | DETEKSI DAN PENENTUAN TIPE BERDASARKAN AMPLIFIKASI DAN SEKUENSING GEN PENYANDI VIRALPROTEIN-2CANINE PARVO VIRUS PADA ANJING SUSPECT CPV |
| title_full | DETEKSI DAN PENENTUAN TIPE BERDASARKAN AMPLIFIKASI DAN SEKUENSING GEN PENYANDI VIRALPROTEIN-2CANINE PARVO VIRUS PADA ANJING SUSPECT CPV |
| title_fullStr | DETEKSI DAN PENENTUAN TIPE BERDASARKAN AMPLIFIKASI DAN SEKUENSING GEN PENYANDI VIRALPROTEIN-2CANINE PARVO VIRUS PADA ANJING SUSPECT CPV |
| title_full_unstemmed | DETEKSI DAN PENENTUAN TIPE BERDASARKAN AMPLIFIKASI DAN SEKUENSING GEN PENYANDI VIRALPROTEIN-2CANINE PARVO VIRUS PADA ANJING SUSPECT CPV |
| title_short | DETEKSI DAN PENENTUAN TIPE BERDASARKAN AMPLIFIKASI DAN SEKUENSING GEN PENYANDI VIRALPROTEIN-2CANINE PARVO VIRUS PADA ANJING SUSPECT CPV |
| title_sort | deteksi dan penentuan tipe berdasarkan amplifikasi dan sekuensing gen penyandi viralprotein 2canine parvo virus pada anjing suspect cpv |
| topic | ETD |
| work_keys_str_mv | AT wahyupeniwijayasti deteksidanpenentuantipeberdasarkanamplifikasidansekuensinggenpenyandiviralprotein2canineparvoviruspadaanjingsuspectcpv AT drdrharisharyantomsi deteksidanpenentuantipeberdasarkanamplifikasidansekuensinggenpenyandiviralprotein2canineparvoviruspadaanjingsuspectcpv |