| Summary: | Periodontal dressing is a dental material which used to cover and protect
the wound after periodontal surgery. Catechins are the chemical compounds found
in green tea leaves and have antibacterial properties. Catechins could be added to
periodontal dressing and able to promote the wound healing on rabbit�s gingiva.
In vitro cytotoxcity assay is used as the preliminary step of material
biocompatibility examinations. The aim of this study was to investigate the
cytotoxic effect of periodontal dressing containing 10% cathecins from green tea
leaves on human primary fibroblast.
Periodontal dressing containing 10% cathecins from green tea leaves
(PDC), periodontal dressing without 10% catechins from green tea leaves (PD),
and Coe-Pak� (CP) were used in this study. Each periodontal dressing was
leached in saliva for seven days. The leach of periodontal dressings were added
into cell suspensions in well-plates, and incubated at 37°C. MTT was added into
well-plate after 24 hours incubation. The cytotoxic effects were measured by
calculting the cell viability (%). Data were analyzed by one way ANOVA,
followed by LSD0.05 test.
The calculation showed that the entire mean values of cell viability were
above 90%. It could be concluded that all of periodontal dressings were classified
as non-cytotoxic on human primary fibroblast. The statistical analysis showed
there were significant differences (p<0.05) between CP and the other groups, but
there was no significant diference between PDC and PD (p>0.05). This study
concluded that periodontal dressing containing 10% cathecins from green tea
leaves had no cytotoxic effect on human primary fibroblast.
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