| Summary: | Bioethanol is an increasingly important alternative biofuel for
transportation. Agricultural biomass are able to be used for the bioethanol
production as raw material. Wheat straw is an abundant agricultural residue and
lignocellulosic feedstock in many parts of the world, it has been selected for
producing ethanol in an economically feasible manner. It contains approximately
40% dry weight cellulose content. In the present study, various lignocellulosic
substrates from wheat straw, i.e. solid fraction, liquid fraction, both of solid and
liquid fractions of pretreated wheat straw and Avicel were examined in
simultaneous saccharification and fermentation (SSF) method for the production
of ethanol by fungi Neurospora intermedia CBS 131.92 and Aspergillus oryzae
var. oryzae CBS 819.72.
The selected filamentous fungi (Neurospora intermedia CBS 131.92 and
Aspergillus oryzae var. oryzae CBS 819.72) were able to produce ethanol from
the solid fraction, the liquid fraction, and the solid-liquid fraction of pretreated
wheat straw. Aspergillus oryzae var. oryzae CBS 819.72 produced higher ethanol
yield than Neurospora intermedia CBS 131.92 from the liquid and the solid
fraction of pretreated wheat straw. Neurospora intermedia CBS 131.92 produced
the highest ethanol yield, i.e. 0.42 g/g glucose consumed from the liquid fraction
of pretreated wheat straw when the initial glucose content was 2.97 g/L.
Aspergillus oryzae var. oryzae CBS 819.72 produced the highest ethanol yield, i.e.
0.40 g/g glucose consumed from the liquid fraction of pretreated wheat straw
when the initial glucose was 3.98 g/L. The highest yield of by-product from SSF
of pretreted wheat straw by Neurospora intermedia CBS 131.92 and Aspergillus
oryzae var. oryzae CBS 819.72 was acetic acid, i.e. 23% on liquid fraction and
46% on liquid fraction, respectively.
Keywords: Pretreated wheat straw
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