| Summary: | The combination of calcitriol and ethinyl ethyl estradiol supplementation
decrease the risk of osteoporosis and endometrial cancer, but it increase calcium
excretion in urine. The objective of this research was to study the effect of
combination calcitriol and ethinyl ethyl estradiol supplementation on
histopathological kidney of Wistar rats ovariectomy. Ten female Wistar rats at 8
weeks of age, were divided into two groups, ovariectomy with ethinyl ethyl
estradiol supplement 0,5 ml/day/rat (OVE) and ovariectomy with a combination
of vitamine D (calcitriol) 0,2 ml/day/rat and ethinyl ethyl estradiol 0,5 ml/day/rat
(OVED), and fed standard diet, and given aquabidestilata ad libitum. Each of rats
was placed into individual cage temperatures 22-25oC. Rats adapted to feed and
the environment for 1 week. Ovariectomy surgery at the age of 9 weeks. One
week after surgery, rats were treated for 8 weeks. After 2 months of treatment, rats
in euthanasia to examination the histopathological of kidney with HE staining. As
a result, ovariectomized rats with ethinyl ehtyl estradiol supplementation showed
no change in kidney. In ovariectomized rats with combination calcitriol and
ethinyl ethyl estradiol supplements showed protein in the glomeruli and proximal
convoluted tubules, atrophy, and necrosis of the proximal convoluted tubule
epithelium. It is concluded that combination calcitriol and ethinyl ethyl estradiol
supplementation in ovariectomized rats causes a nephrosis.
Keywords: ovariectomy, histopathology kidney, ethinyl ethyl estradiol, calcitriol
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