| Summary: | Lowo (Myotis sp.) is one at Indonesia wild animals that belongs to the
class Mammalians, order Chiroptera, suborder Microchiroptera and family
Vespertilionidae. Thus, it is one of the bats from suborder Microchiroptera that
feed on insect. The duodenum has two main function, to digest food as a part of
the small intestine, and absorb nutrition from the digested food. This unique
feeding behavior of lowo is the reason behind this research about the histology of
lowo�s duodenum.
In this study, five lowo�s duodenum from Magelang were collected.
Tissues were processed with parafin method, then cut in 5-6ï�m thickness and
mounted in coated slide. Tissues inside the coated slide are then stained using the
Hematoxylin Eosine (HE) and Alcian Blue pH 2,5-Periodic Acid Schiff (AB pH
2,5-PAS). The tissue were examide microscopically and is analyzed descriptively.
Based on the observation, it can be concluded that the histological
structure of lowo�s duodenum is composed of tunica mucosa, tunica submucosa,
tunica muscularis, and tunica serosa. Brunner glands� characteristic are serous,
distributed from the end of pyloric sphingter to the beginning of the duodenum.
The average length of each gland is 686,5 + 89,62 ï�m. Goblet cells are distributed
in the lamina epithelial mucosae and lieberkuhn crypt, and they are react
positively to the Alcian blue pH 2,5 staining, while the absorbtion cells, columnar
cells in the lieberkuhn crypt, and Brunner glands react positively to Periodic Acid
Schiff staining. The distribution of acid mucopolysaccharides are found in the
goblet cells in lamina epithelial mucosae and goblet cells of lieberkuhn crypt.
Neutral muccopolysaccharides are found in the absorbtion cells, columnar cells of
lieberkuhn crypt, and Brunner glands in tunica submucosa.
Keywords : HE, AB-PAS, lowo, duodenum, Brunner glands,
muccopolysaccharides.
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