| Summary: | Periodontal dressing was developed with some additives which aimed to
increase its value from a mechanical wound barrier to a wound healing agent.
Fibroblast proliferation as one of the wound healing indicator need to be assessed
further. One of the natural compound with wound healing potential was green tea
catechins but recent studies about catechins effects regarding fibroblast
proliferation had variety of results, wether enhanced or suppressed fibroblast
proliferation. This study aimed to attain the effect of 10% green tea catechins
addition in periodontal dressing on human primary fibroblast proliferation.
This study used 3 types of periodontal dressing: Baer�s periodontal dressing
(PD), Baer�s periodontal dressing with 10% green tea catechins addition (PDC),
and Coe-PakTM (CP). Each periodontal dressing was immersed in natural saliva
for one day then added to human primary fibroblast culture in the microplate and
incubated for 24 and 72 hours. After the incubation period, proliferation test
conducted with MTT assay which resulted optical density (OD) value. Data then
analyzed by MANOVA, LSD, and Independent T Test.
Statistical analysis of OD value after 24 hours incubation showed that there
were no significant differences (p>0,05) among CP, PD, and PDC whereas data of
72 hours incubation showed significant differences (p<0,05). OD value of PDC
was lower than CP and PD while there was no significant difference (p>0,05)
between CP and PD. In conclusion, 10% green tea catechins addition in
periodontal dressing inhibited human primary fibroblast proliferation.
Keywords: Green tea catechins, periodontal dressing, proliferation, human
primary fibroblast
|
|---|