Summary: | Analysis technology of pesticide residues more develop in a row with
increases usage pesticide in a field. There are several of residues analysis method,
one of them is HPLC.
This research aims to perform validate posseses of analysis deltamethrin
using a Shimadzu 6.1 high-performance liquid chromatography (HPLC) with control
system SCL-10 A VP, DGU-14 A degasser. Mobile phase of Merck acetonitrile 80%
in steryl aquadest, flow rate of 1.25 ml/minute, UV-Vis SPD 10 AV VP detector with
wavelength of 236 nm and used Shimadzu Shim-pack 150 L x 4.6 mm VP-ODS
Column C18 on temperature 30 oC. The validation examination is based on
specificity, precision, accuracy, limit of qualitative, quantitative limits, and linearity.
Results of the research shows the average of area concentration 1 μg/ml,
2 μg/ml, and 5 μg/ml in consecutive are 34620.33, 79048.67, dan 181208.7 with
retention time in 8.6-8.98 minute. Repeatability is clone 3 times and the RSD mean
value obtained is � 2%. The detection limit at 0.1 μg/ml and limit of quantification at
concententration 1 μg/ml. The equation of linear line y = 36046x+2182, with the
value of R= 0.99. It can be concluded that the method used in the research posseses
good validity.
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