| Summary: | Background: Cancer is the leading cause of death in the world . Potential natural
materials to be developed as anticancer compounds includes flavonoids group.
Isoflavones are one of the flavonoid compounds. Isoflavones have various
pharmacological activities such as antioxidant, anti-inflammatory, antimutagenic,
and anticancer. This study was conducted to determine the expression of cox - 2
in the liver of Spraque Dawley rats exposed to1,2 epoxy - 3 - [ 3 - ( 3,4 -
dimetoksifenil ) 4H - 1 - benzopyran - 4-one ] propane ( EPI ) and the induction of
DMBA. Expression of COX - 2 is one of the signs of inflammation and is a major
contributing factor in carcinogenesis.
Objective: To determine the expression of COX - 2 Spraque -Dawley rats liver
cells that have been induced by DMBA and EPI.
Methods: Thirty- six rats were divided into 6 groups. Every 3 days was
conducted clinical observation namely body weight and viability on the test
animals. Rats were given 20mg/kg DMBA body weight dissolved in corn oil and
doses of 1 mg, 2 mg and 4 mg / kg BW of EPI compounds. DMBA and corn oil
injection were conducted twice a week for 5 weeks. Administration of food and
drink equated for all groups namely AD II standard feed and ad libitum aqua
drinking water. Observation conducted for 15 weeks, after which the rats were
sacrificed and sent to the histology preparations for the manufacture of paraffin
blocks and IHC coloring with monoclonal antibody anti- COX-2 rats. Histological
observation of the liver of rats aimed to see the expression of COX-2. Calculation
of COX-2 expressions was measured using a light microscope at 5 visual fields
with 40x magnification. COX-2 expression was characterized by the brown color
in the cytoplasm cell. The average yield from each group was analyzed by
ANOVA parametric statistical methods.
Results: Normal group with the corn oil group, 1 mg EPI, 2 mg and 4 mg did not
differ significantly (p > 0.05). DMBA group expressed most COX - 2. Rat liver of
EPI group expressed COX - 2 lower than the DMBA group (p < 0.05).
Conclusion: Administration of EPI compounds was able to reduce the expression
of COX - 2 in liver cells Spraque -Dawley rats that had received exposure to
DMBA induction
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