ANALISIS KURKUMIN DALAM EKSTRAK ETANOLIK TEMULAWAK (Curcuma xanthorrhiza Roxb.) SECARA SPEKTROFOTOMETRI INFRAMERAH YANG DIKOMBINASIKAN DENGAN KALIBRASI MULTIVARIAT
Levels of curcumin in turmeric rhizome needs to be analyzed, to control the quality of ginger. The method of analysis using chromatographic curcumin generally tend to take a long time and requires skilled personnel to perform the analysis. The purpose of this research is to gain a practical and effi...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| Summary: | Levels of curcumin in turmeric rhizome needs to be analyzed, to control the quality of ginger. The method of analysis using chromatographic curcumin generally tend to take a long time and requires skilled personnel to perform the analysis. The purpose of this research is to gain a practical and efficient method for the analysis of curcumin in ethanolic extract of ginger. Samples of approximately 50 mg of a number of carefully made into powder and macerated with 70% ethanol. Extracts were analyzed by Fourier Transform Infrared Spectrophotometer (FTIR) on wave number 4000-650 cm-1, while curcumin content was quantified by the method of High Performance Liquid Chromatography (HPLC) as the reference standard method. Analysis was performed on wave number region 2000 - 950 cm-1. Calibration model Partial Least Square (PLS) in the form y = 0.979 x + 0.000005 showed good correlation to describe the concentration of curcumin predictable FTIR spectrophotometer (y) the actual concentration of curcumin were analyzed by HPLC (x), with a coefficient of determination (R2 ) value of 0.995 and Root Mean Square Error of Calibration (RMSEC) of 0.008%. R2 value close to 1 indicates that the FTIR spectrophotometer combined with multivariate calibration (PLS) as curcumin is capable of analyzing quantitative HPLC method. |
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