PENGARUH METFORMIN TERHADAP PENEKANAN EKSPRESI CYCLIN D1, AKTIVASI p53 DAN INDUKSI APOPTOSIS PADA KULTUR SEL HELA
Background : Indonesia is a country with the highest number of cervical cancer in the world.Chemotherapy for cancer is less favorable and often causes serious side effects. Metformin is widely used as antidiabetic agents. Recent studiesfoundthat metformin had the potentialanticancer effect. This stu...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| author | , RATIH DEWI YUDHANI , Dr. Med.dr. Indwiani Astuti |
| author_facet | , RATIH DEWI YUDHANI , Dr. Med.dr. Indwiani Astuti |
| author_sort | , RATIH DEWI YUDHANI |
| collection | UGM |
| description | Background : Indonesia is a country with the highest number of cervical cancer in the
world.Chemotherapy for cancer is less favorable and often causes serious side effects.
Metformin is widely used as antidiabetic agents. Recent studiesfoundthat metformin had the
potentialanticancer effect. This study to determine the anticancer effect of metformin on
HeLa cell, as an attempt to discover and develop novel therapeutic strategies in the fight
against cancer, particularly cervical cancer.
Objective : To asses the potential anticancer effect of metfomin on HeLa cell and
specifically to determine the metformin activity on cyclin D1suppression, p53 activation
and apoptosis induction in HeLa cell cultures.
Methods :Cytotoxic test with MTT assay was performed to determine IC50 metformin in
HeLa cells. Observationcyclin D1 suppression and p53 activation by metformin were
peformed using immunocytochemistry, whereas apoptosis induction test was done by
flowcytometry.
Results : Metforminhasa tendencyto inhibitproliferationof HeLa cellswithIC50
59,62mM.Metformin30, 60, and120mMsuppressed cyclinD1expression,activated p53and
induced apoptosis in HeLa cellsina dose-dependent manner.
Conclusion: Metformin has a tendency to inhibitproliferation and induce apoptosis in HeLa
cells culture in a dose-dependent manner. This activity of metformin was associated with its
ability to suppress cyclin D1 expression and activation of p53. |
| first_indexed | 2024-03-13T23:33:25Z |
| format | Thesis |
| id | oai:generic.eprints.org:131559 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:33:25Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1315592016-03-04T08:08:21Z https://repository.ugm.ac.id/131559/ PENGARUH METFORMIN TERHADAP PENEKANAN EKSPRESI CYCLIN D1, AKTIVASI p53 DAN INDUKSI APOPTOSIS PADA KULTUR SEL HELA , RATIH DEWI YUDHANI , Dr. Med.dr. Indwiani Astuti ETD Background : Indonesia is a country with the highest number of cervical cancer in the world.Chemotherapy for cancer is less favorable and often causes serious side effects. Metformin is widely used as antidiabetic agents. Recent studiesfoundthat metformin had the potentialanticancer effect. This study to determine the anticancer effect of metformin on HeLa cell, as an attempt to discover and develop novel therapeutic strategies in the fight against cancer, particularly cervical cancer. Objective : To asses the potential anticancer effect of metfomin on HeLa cell and specifically to determine the metformin activity on cyclin D1suppression, p53 activation and apoptosis induction in HeLa cell cultures. Methods :Cytotoxic test with MTT assay was performed to determine IC50 metformin in HeLa cells. Observationcyclin D1 suppression and p53 activation by metformin were peformed using immunocytochemistry, whereas apoptosis induction test was done by flowcytometry. Results : Metforminhasa tendencyto inhibitproliferationof HeLa cellswithIC50 59,62mM.Metformin30, 60, and120mMsuppressed cyclinD1expression,activated p53and induced apoptosis in HeLa cellsina dose-dependent manner. Conclusion: Metformin has a tendency to inhibitproliferation and induce apoptosis in HeLa cells culture in a dose-dependent manner. This activity of metformin was associated with its ability to suppress cyclin D1 expression and activation of p53. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , RATIH DEWI YUDHANI and , Dr. Med.dr. Indwiani Astuti (2014) PENGARUH METFORMIN TERHADAP PENEKANAN EKSPRESI CYCLIN D1, AKTIVASI p53 DAN INDUKSI APOPTOSIS PADA KULTUR SEL HELA. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=72055 |
| spellingShingle | ETD , RATIH DEWI YUDHANI , Dr. Med.dr. Indwiani Astuti PENGARUH METFORMIN TERHADAP PENEKANAN EKSPRESI CYCLIN D1, AKTIVASI p53 DAN INDUKSI APOPTOSIS PADA KULTUR SEL HELA |
| title | PENGARUH METFORMIN TERHADAP PENEKANAN EKSPRESI CYCLIN D1, AKTIVASI p53 DAN INDUKSI APOPTOSIS PADA KULTUR SEL HELA |
| title_full | PENGARUH METFORMIN TERHADAP PENEKANAN EKSPRESI CYCLIN D1, AKTIVASI p53 DAN INDUKSI APOPTOSIS PADA KULTUR SEL HELA |
| title_fullStr | PENGARUH METFORMIN TERHADAP PENEKANAN EKSPRESI CYCLIN D1, AKTIVASI p53 DAN INDUKSI APOPTOSIS PADA KULTUR SEL HELA |
| title_full_unstemmed | PENGARUH METFORMIN TERHADAP PENEKANAN EKSPRESI CYCLIN D1, AKTIVASI p53 DAN INDUKSI APOPTOSIS PADA KULTUR SEL HELA |
| title_short | PENGARUH METFORMIN TERHADAP PENEKANAN EKSPRESI CYCLIN D1, AKTIVASI p53 DAN INDUKSI APOPTOSIS PADA KULTUR SEL HELA |
| title_sort | pengaruh metformin terhadap penekanan ekspresi cyclin d1 aktivasi p53 dan induksi apoptosis pada kultur sel hela |
| topic | ETD |
| work_keys_str_mv | AT ratihdewiyudhani pengaruhmetforminterhadappenekananekspresicyclind1aktivasip53daninduksiapoptosispadakulturselhela AT drmeddrindwianiastuti pengaruhmetforminterhadappenekananekspresicyclind1aktivasip53daninduksiapoptosispadakulturselhela |