| Summary: | Worms Paratanaisia bragaiare classified into trematode worms which are
predilected in pigeon�s kidney (Columba livia). The purpose of this paper is to find
out how Paratanaisia bragai staining worms with the use ofSemichon's carmine
staining method. Analysis of Paratanaisia bragaiworm staining process is presented
descriptively. Staining method which is suitable for Paratanaisia bragai worm is
theSemichon's carmine staining. Staining was conducted by soaking worms in a
solution of AFA (Alcohol Formal Acetic Acid) for 24 hours, dehydratingthem using
multilevelalcohol each for 15 minutes, soakingthem in dye Semichon's carmine for 1
hour, washingthem with 70% alcohol for 15 minutes, spilling HCL-alcohol 0.5-1%,
dehydrating themwith multilevel alcohol, and soaking them in xylol for 1 minute.
Staining results were observed with the use of a stereo microscope, the worm seems
to have the suction mulit vanity, testis, pharynx, spine, ovary, and vitelaria. It can be
concluded that the immersion time in the solution should be done precisely to obtain
optimum results. Wormtissue damage is caused by crushing kidney too strongly,
wormbody soaking in each solution are not done exhaustively, and the length of time
soaking worms in a solution which is not precise.
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