PENGARUH KONSENTRASI EKSTRAK KULIT BATANG JAMBU METE SEBAGAI DASAR BAHAN KUMUR TERHADAP SITOTOKSISITAS SEL FIBROBLAS DENGAN METODE MTT
Recurrent aphthous stomatitis is an inflammation that occurs in the oral mucosa. One of the treatment to cure recurrent aphthous stomatitis is by using mouthwash ingredients. Cashew bark contains phenolic compounds that include flavonoids, tannins and saponins. The aim of this study was to determine...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| _version_ | 1826048736856375296 |
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| author | , AHMED FEBRI NOOR HERTAMA , Drg. Harsini, M.S. |
| author_facet | , AHMED FEBRI NOOR HERTAMA , Drg. Harsini, M.S. |
| author_sort | , AHMED FEBRI NOOR HERTAMA |
| collection | UGM |
| description | Recurrent aphthous stomatitis is an inflammation that occurs in the oral
mucosa. One of the treatment to cure recurrent aphthous stomatitis is by using
mouthwash ingredients. Cashew bark contains phenolic compounds that include
flavonoids, tannins and saponins. The aim of this study was to determine the effect of
variations in the concentration of cashew stem bark extract as the base material
mouthwash towards cytotoxic effect on fibroblast cells using MTT method.
This material used in this study was cashew stem bark which were extracted
by maceration method using 70% ethanol. A total of 15 samples were grouped into 5,
each consisting of 3 samples (ISO 10993-5). Concentrations used were 1.6%, 0.8%,
0.4%, 0.2% dan 0.05%. Cytotoxicity test uses the MTT method by comparing optical
density (ELISA plate reader) between treated groups with control groups. Cell
viability was obtained by comparing treated groups with control groups. Cell viability
data was analyzed using one way ANOVA and LSD.
Results showed mean of fibroblast cell viability due to various cashew stem
bark extract successively from concentrations 1.6%, 0.8%, 0.4%, 0.2% dan 0.05%.
With mean 15.35 ± 0.443%, 30.84% ± 1.59, 47.78 ± 8.09%, 65.74% ± 3.20, 74.95 ±
7.26%. ANOVA showed a significant influence various cashew stem bark on cell
viability (p<0,05). Results of LSD showed there were significant difference between
treated groups except between concentrations 0.2% and 0.05%. In conclusion, there
was influence on the various cashew stem bark extract concentrations towards
fibroblast cell cytotoxicity. |
| first_indexed | 2024-03-13T23:34:51Z |
| format | Thesis |
| id | oai:generic.eprints.org:132002 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:34:51Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1320022016-03-04T07:51:52Z https://repository.ugm.ac.id/132002/ PENGARUH KONSENTRASI EKSTRAK KULIT BATANG JAMBU METE SEBAGAI DASAR BAHAN KUMUR TERHADAP SITOTOKSISITAS SEL FIBROBLAS DENGAN METODE MTT , AHMED FEBRI NOOR HERTAMA , Drg. Harsini, M.S. ETD Recurrent aphthous stomatitis is an inflammation that occurs in the oral mucosa. One of the treatment to cure recurrent aphthous stomatitis is by using mouthwash ingredients. Cashew bark contains phenolic compounds that include flavonoids, tannins and saponins. The aim of this study was to determine the effect of variations in the concentration of cashew stem bark extract as the base material mouthwash towards cytotoxic effect on fibroblast cells using MTT method. This material used in this study was cashew stem bark which were extracted by maceration method using 70% ethanol. A total of 15 samples were grouped into 5, each consisting of 3 samples (ISO 10993-5). Concentrations used were 1.6%, 0.8%, 0.4%, 0.2% dan 0.05%. Cytotoxicity test uses the MTT method by comparing optical density (ELISA plate reader) between treated groups with control groups. Cell viability was obtained by comparing treated groups with control groups. Cell viability data was analyzed using one way ANOVA and LSD. Results showed mean of fibroblast cell viability due to various cashew stem bark extract successively from concentrations 1.6%, 0.8%, 0.4%, 0.2% dan 0.05%. With mean 15.35 ± 0.443%, 30.84% ± 1.59, 47.78 ± 8.09%, 65.74% ± 3.20, 74.95 ± 7.26%. ANOVA showed a significant influence various cashew stem bark on cell viability (p<0,05). Results of LSD showed there were significant difference between treated groups except between concentrations 0.2% and 0.05%. In conclusion, there was influence on the various cashew stem bark extract concentrations towards fibroblast cell cytotoxicity. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , AHMED FEBRI NOOR HERTAMA and , Drg. Harsini, M.S. (2014) PENGARUH KONSENTRASI EKSTRAK KULIT BATANG JAMBU METE SEBAGAI DASAR BAHAN KUMUR TERHADAP SITOTOKSISITAS SEL FIBROBLAS DENGAN METODE MTT. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=72517 |
| spellingShingle | ETD , AHMED FEBRI NOOR HERTAMA , Drg. Harsini, M.S. PENGARUH KONSENTRASI EKSTRAK KULIT BATANG JAMBU METE SEBAGAI DASAR BAHAN KUMUR TERHADAP SITOTOKSISITAS SEL FIBROBLAS DENGAN METODE MTT |
| title | PENGARUH KONSENTRASI EKSTRAK KULIT BATANG
JAMBU METE SEBAGAI DASAR BAHAN KUMUR
TERHADAP SITOTOKSISITAS SEL FIBROBLAS
DENGAN METODE MTT |
| title_full | PENGARUH KONSENTRASI EKSTRAK KULIT BATANG
JAMBU METE SEBAGAI DASAR BAHAN KUMUR
TERHADAP SITOTOKSISITAS SEL FIBROBLAS
DENGAN METODE MTT |
| title_fullStr | PENGARUH KONSENTRASI EKSTRAK KULIT BATANG
JAMBU METE SEBAGAI DASAR BAHAN KUMUR
TERHADAP SITOTOKSISITAS SEL FIBROBLAS
DENGAN METODE MTT |
| title_full_unstemmed | PENGARUH KONSENTRASI EKSTRAK KULIT BATANG
JAMBU METE SEBAGAI DASAR BAHAN KUMUR
TERHADAP SITOTOKSISITAS SEL FIBROBLAS
DENGAN METODE MTT |
| title_short | PENGARUH KONSENTRASI EKSTRAK KULIT BATANG
JAMBU METE SEBAGAI DASAR BAHAN KUMUR
TERHADAP SITOTOKSISITAS SEL FIBROBLAS
DENGAN METODE MTT |
| title_sort | pengaruh konsentrasi ekstrak kulit batang jambu mete sebagai dasar bahan kumur terhadap sitotoksisitas sel fibroblas dengan metode mtt |
| topic | ETD |
| work_keys_str_mv | AT ahmedfebrinoorhertama pengaruhkonsentrasiekstrakkulitbatangjambumetesebagaidasarbahankumurterhadapsitotoksisitasselfibroblasdenganmetodemtt AT drgharsinims pengaruhkonsentrasiekstrakkulitbatangjambumetesebagaidasarbahankumurterhadapsitotoksisitasselfibroblasdenganmetodemtt |