PENINGKATAN SENSITIVITAS SEL KANKER KOLON WiDr TERHADAP 5-FLUOROURASIL OLEH FRAKSI ETIL ASETAT KAYU SECANG (Caesalpinia sappan L.) MELALUI INDUKSI APOPTOSIS
Continuous administration of chemotherapy in cancer cells could decrease its sensitivity to chemotherapeutic agent. Strategy to overcome this problem is cochemotherapy with chemoprevention agent which has synergist effect with chemotherapeutic agent to eradicate cancer cells. On this research, we st...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| _version_ | 1826048767586992128 |
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| author | , ANNISA NOVARINA , Dr. Riris Istighfari Jenie. |
| author_facet | , ANNISA NOVARINA , Dr. Riris Istighfari Jenie. |
| author_sort | , ANNISA NOVARINA |
| collection | UGM |
| description | Continuous administration of chemotherapy in cancer cells could decrease its
sensitivity to chemotherapeutic agent. Strategy to overcome this problem is cochemotherapy
with chemoprevention agent which has synergist effect with
chemotherapeutic agent to eradicate cancer cells. On this research, we studied ethyl
acetate fraction of Caesalpinia sappan L. (EFC) as co-chemotherapeutic agent. EFC
was characterized using thin layer chromatography (TLC). Cytotoxic effect of EFC
was examined using MTT assay, apoptosis detection using double staining assay, and
we observed expression of cleaved PARP as apoptotic protein marker using
immunocytochemistry. Potency of EFC and 5-Fluorouracil (5-FU) against WiDr cell
lines was identified through IC50 value. Effect of combination 5-FU and EFC was
determined form combination index (CI) as parameter of synergist effect from this
combination. Apoptosis induction was determined through result from double
staining assay and expression of cleaved PARP.
Visual TLC profile of EFC with system consist of n-heksan, kloroform,
metanol, asam asetat glasial (1:7:2:1) as mobile phase and silika F254 as stationary
phase showed EFC contain brazilein as major compound with hRf 81. EFC could
inhibit WiDr cancel cells growth through decrease of cells viability and morphology
changing. IC50 value of EFC is 11 μg/mL in dose dependent manner. 5-FU as
chemotherapeutic agent for colon cancer showed cytotoxic effect with IC50 558 μM.
Combination of EFC and 5-FU showed synergist effect with CI value 0,3 at dose ½
IC50 of EFC and ½ IC50 of 5-FU. Synergist effect of this combination is predicted
through apoptosis induction that showed in double staining assay result. Apoptosis
mechanism was showed through decrease expression of cleaved PARP. |
| first_indexed | 2024-03-13T23:35:20Z |
| format | Thesis |
| id | oai:generic.eprints.org:132158 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:35:20Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1321582016-03-04T07:52:22Z https://repository.ugm.ac.id/132158/ PENINGKATAN SENSITIVITAS SEL KANKER KOLON WiDr TERHADAP 5-FLUOROURASIL OLEH FRAKSI ETIL ASETAT KAYU SECANG (Caesalpinia sappan L.) MELALUI INDUKSI APOPTOSIS , ANNISA NOVARINA , Dr. Riris Istighfari Jenie. ETD Continuous administration of chemotherapy in cancer cells could decrease its sensitivity to chemotherapeutic agent. Strategy to overcome this problem is cochemotherapy with chemoprevention agent which has synergist effect with chemotherapeutic agent to eradicate cancer cells. On this research, we studied ethyl acetate fraction of Caesalpinia sappan L. (EFC) as co-chemotherapeutic agent. EFC was characterized using thin layer chromatography (TLC). Cytotoxic effect of EFC was examined using MTT assay, apoptosis detection using double staining assay, and we observed expression of cleaved PARP as apoptotic protein marker using immunocytochemistry. Potency of EFC and 5-Fluorouracil (5-FU) against WiDr cell lines was identified through IC50 value. Effect of combination 5-FU and EFC was determined form combination index (CI) as parameter of synergist effect from this combination. Apoptosis induction was determined through result from double staining assay and expression of cleaved PARP. Visual TLC profile of EFC with system consist of n-heksan, kloroform, metanol, asam asetat glasial (1:7:2:1) as mobile phase and silika F254 as stationary phase showed EFC contain brazilein as major compound with hRf 81. EFC could inhibit WiDr cancel cells growth through decrease of cells viability and morphology changing. IC50 value of EFC is 11 μg/mL in dose dependent manner. 5-FU as chemotherapeutic agent for colon cancer showed cytotoxic effect with IC50 558 μM. Combination of EFC and 5-FU showed synergist effect with CI value 0,3 at dose ½ IC50 of EFC and ½ IC50 of 5-FU. Synergist effect of this combination is predicted through apoptosis induction that showed in double staining assay result. Apoptosis mechanism was showed through decrease expression of cleaved PARP. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , ANNISA NOVARINA and , Dr. Riris Istighfari Jenie. (2014) PENINGKATAN SENSITIVITAS SEL KANKER KOLON WiDr TERHADAP 5-FLUOROURASIL OLEH FRAKSI ETIL ASETAT KAYU SECANG (Caesalpinia sappan L.) MELALUI INDUKSI APOPTOSIS. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=72677 |
| spellingShingle | ETD , ANNISA NOVARINA , Dr. Riris Istighfari Jenie. PENINGKATAN SENSITIVITAS SEL KANKER KOLON WiDr TERHADAP 5-FLUOROURASIL OLEH FRAKSI ETIL ASETAT KAYU SECANG (Caesalpinia sappan L.) MELALUI INDUKSI APOPTOSIS |
| title | PENINGKATAN SENSITIVITAS SEL KANKER KOLON WiDr TERHADAP 5-FLUOROURASIL OLEH FRAKSI ETIL ASETAT KAYU SECANG (Caesalpinia sappan L.) MELALUI INDUKSI APOPTOSIS |
| title_full | PENINGKATAN SENSITIVITAS SEL KANKER KOLON WiDr TERHADAP 5-FLUOROURASIL OLEH FRAKSI ETIL ASETAT KAYU SECANG (Caesalpinia sappan L.) MELALUI INDUKSI APOPTOSIS |
| title_fullStr | PENINGKATAN SENSITIVITAS SEL KANKER KOLON WiDr TERHADAP 5-FLUOROURASIL OLEH FRAKSI ETIL ASETAT KAYU SECANG (Caesalpinia sappan L.) MELALUI INDUKSI APOPTOSIS |
| title_full_unstemmed | PENINGKATAN SENSITIVITAS SEL KANKER KOLON WiDr TERHADAP 5-FLUOROURASIL OLEH FRAKSI ETIL ASETAT KAYU SECANG (Caesalpinia sappan L.) MELALUI INDUKSI APOPTOSIS |
| title_short | PENINGKATAN SENSITIVITAS SEL KANKER KOLON WiDr TERHADAP 5-FLUOROURASIL OLEH FRAKSI ETIL ASETAT KAYU SECANG (Caesalpinia sappan L.) MELALUI INDUKSI APOPTOSIS |
| title_sort | peningkatan sensitivitas sel kanker kolon widr terhadap 5 fluorourasil oleh fraksi etil asetat kayu secang caesalpinia sappan l melalui induksi apoptosis |
| topic | ETD |
| work_keys_str_mv | AT annisanovarina peningkatansensitivitasselkankerkolonwidrterhadap5fluorourasilolehfraksietilasetatkayusecangcaesalpiniasappanlmelaluiinduksiapoptosis AT drririsistighfarijenie peningkatansensitivitasselkankerkolonwidrterhadap5fluorourasilolehfraksietilasetatkayusecangcaesalpiniasappanlmelaluiinduksiapoptosis |