| Summary: | The production of embryos using in vitro method which consists of
maturation, fertilisationand culture needs qualified oocytes in order to get good
embryos. The size of ovarian, the producer of oocytes, which is taken from abbatoir
(RPH) is vary from one individu to another. To fulfill the need of oocytes from the
abattoir (RPH), ovarian as the primary material required precise and rapid handling.
The indirect selection of oocytes could be conduct by examining other organs which
have relation to the oocytes quality, and in this research examining the size of the
ovarian was the selected way. The purpose of this research was to find out the effects
of the size of cow�s ovarian to the quality of oocytes morphology.
The ovarians taken from abattoir (RPH) were washed using 0,9%
phyciological Nacl which had been added with antibiotics Penicilin 100 IU/ml and
Streptomycin 100 μg/ml. Then, the ovarians were classified based on their size. The
first group consists of1,0 � 1,9 cm, the second group consists of 2,0 � 2,9 cm and the
third group consists of 3,0 � 3,9 cm. Oocytes were collected using aspiration method
which used 10 ml spuit and 18 G needle. The observation of the oocytes collection
was conduct using stereo microscope and were classified based on the oocytes
morphology. The collected data were analyzed using ANOVA.
The findings of the research is that quality of oocytes morphology do not have
significant variation to the size of cow�s ovarians. The second group produced
oocytes with A 34, 25% and B 41,10% quality which was higher than the first group
(22,33% and 43, 69%) and the third group (16,53 % and 32,23%). Meanwhile, for the
amount of the oocytes A and B quality is higher in the second group (75, 35%). From
the data, it can be conclude that the size of cow�s ovarian does not influence to the
procentage of the amount of oocytes based on the quality of morphology of A and B
oocytes.
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