| Summary: | Neutrophils and fibroblasts play important role in wound healing process.
Neutrophils as phagocytic cell functioned to avoid bacterial invasion in wounded
tissues. In wound healing process, fibroblasts provide collagen those very
important in regeneration of damaged tissues. This study aimed to know the
effect of treatment with turmeric (Curcuma domestica Val) toward wound healing
process represented by fibroblast cells and neutrophils of incisive wound of skin
in dog (Canine familiaris).
A total of four dogs used in this study. On the right flank and left of each
dog made three incisions to fascia layer with 6 cm long. The space between
incisions is 3 cm, then the wound sutured with cotton, then treated with control,
fresh turmeric and turmeric powder. This treatment applied twice a day in a week.
On the third and sixth day, the wounded tissues were prepared for histological
examination with Hematoksilin-Eosin(HE) and Mallory staining. Data are
collected from neutrophils and fibroblasts count using light microscope 400x in 5
fields. Data analyzed with one way ANOVA method and then with Tukey HSD.
The results of this study show that treatment with turmeric (Cucuma
domestica Val) is capable to improve neutrophils and fibroblasts. Increase in the
number of neutrophils in wound assumed as the effect of NRF increase, while
increase of fibroblast count was estimated as the effect of TGF- β1 increase that
functioned to induce migration of fibroblast to wounded tissues.
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