| Streszczenie: | Infectious bursal disease (IBD) is a viral disease that caused by double
strand RNA (dsRNA) virus that derived from Birnaviridae family. The disease is
well known as Gumboro has infected lymphoid tissue of 3-6 weeks old young
chicken, especially on bursa of Fabrisius. Gumboro cause a huge economic loss in
the poultry industry, examples increasing the mortality and morbidity in chickens
due to the immunosuppressive effects, therefore, early detection of IBD virus is
necessary to prevent spreading of IBDV. A moleculer techniques that have been
frequently used to detect IBDV is Reverse Transcriptase Polymerase Chain
Reaction (RT-PCR), RT-PCR method was choosen due to highly sensitifity and
accurate than the convensional methods such as ELISA and AGPT. This research
aims to identify IBDV with one-step RT-PCR method.
The tested tissue used in this research were isolate IBD virus
chorioallantois membrane (MHW/Yanti/Layer/2011 isolate and MHW/Bro/2012
isolate) and samples of bursa of Fabrisius (MHW/SR-A, MHW/SR-B,
MHW/N18/15-4-2014, MHW/N18/14-4-2014, MWH/N1/15-4-2014 and
MHW/NF/2014). Firstly, the samples dan isolates were made into suspension,
then the suspension were extracted to obtain RNA using Extraction RNA kit from
GeneAid and then perform the RT-PCR using RT-PCR kit Invitrogen with the
forward primer (5´-GTCTACACCATAACTGCCGCAGATGAT-3´) and reverse
primer (5´-GGCTACTAGTGTGACGGGGCGGAGGGCACC-3´) that will
amplify VP2 gene of IBD virus and giving product of 440 bp. The results of RTPCR
were read using 1.5% agarose gel electrophoresis.
The results of thus research showed two isolates (MHW/Yanti/Layer/2011
isolate and MHW/Bro/2012 isolate) were positive infected by IBDV while six
samples were negative (MHW/SR-A, MHW/SR-B, MHW/N18/15-4-2014,
MHW/N18/14-4-2014, MWH/N1/15-4-2014 and MHW/NF/2014). Positive
results on agarose gel electrophoresis was showed by giving product of 440bp.
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