| Summary: | Lead (Pb) is a heavy metal which is widely used by human in some
industries, especially batteries, power tools, and printing industries. It is also used
in gasoline, paints and pesticides. This metal is an environmental pollutant
(multimedia pollutant) and the main source of soil contamination Water spinach
(Ipomoea reptans Poir.) are known to absorb heavy metals through their roots.
The purpose of this study was to study the anatomical, physiological, and
morphological responses of Ipomoea reptans Poir. to lead. Research was carried
out by treating plants with Pb (NO3)2 60, 80, 100, and 120 ppm along with a
negative control at 10 days old plant. Parameters observed were stem diameter,
epidermis cells thickness, cortex thickness, trachea thickness, stele thickness, pith
cavity diameter, levels of chlorophyll a, chlorophyll b, total chlorophyll, lead
concentrations in the roots, stems, leaves, plant height, number of leaves, and ratio
of the length and with. Data were analyzed by ANOVA followed by DMRT with
5% of significance level.
The results showed that 120 ppm Pb affects the anatomical structure of the
stem by reducing stem diameter, trachea cells diameter, cortex thickness, stele
thickness and pith cavity diameter. Otherwise, 120 ppm of Pb increased epidermal
cells thickness. Pb concentration up to 120 ppm reduced chlorophyll a, b, and total
content. Pb was accumulated in small amounts in the roots plant treated with 60
and 80 ppm of Pb. Pb did not affect the height plant, number of leaves, and the
ratio of the length and width of plant leaves. From these results it can be
concluded that the adsorbed Pb can affect the epidermal cells thickness, diameter
of stem, trachea, pith cavity, cortex and stele tissues. Pb affect chlorophyll a, b, c
and total of I. reptans but has not been able to affect plant morphology.
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