| Summary: | Kaffir lime (Citrus hystrix D.C) is an herbal plant which widely spread in
Indonesia. During this time, the use of plants is still limited as food ingredients
and aromatherapy oils. Leaves of C. hystrix contain flavonoids, alkaloids,
saponins, polyphenols, terpenoids, tannins, and essential oils that are cytotoxic to
leuchemic T-cell and cervical cancer cell line. Objectives of this study were to
determine cytotoxicity and apoptotic induction of C. hystrix leaves extract on
T47D cells and Vero cells. Methods that used in study were maceration using
three solvents (ethyl acetate, chloroform, and ethanol 96%), cytotoxic assay by
MTT assay, apoptotic assay using double staining (ethidium bromide-acrydine
orange). The results showed that cytotoxicity assay of chloroform extract (IC50=
128,89 μg/mL ) have the highest cytotoxic activity on T47D cells compare to
ethyl acetate extract (IC50= 283,12 μg/mL) and ethanolic extract (IC50= 444
μg/mL). Whereas in Vero cells three extract were not reached IC50 hence these
extract had no cytotoxic effect in non-cancer cells. Furthermore apoptotic rate of
extract chloroform, ethyl acetat, and ethanol 96% were (51,45%), (31,47%),
(36.24%) respectively. Chloroform extract kaffir lime leaves most potential to
induce apoptosis of T47D cells followed by ethyl acetat and ethanol.
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