| Izvleček: | The methane oxidation activity by methane-oxidizing bacteria that occur in
paddy fields can be influenced by fertilizer addition. Generally, fertilization in paddy
fields are using nitrogen-based fertilizer (i.e. nitrogen source), the presence of
nitrogen source in an ecosystem is considered to be very important, because its
presence can influence methane oxidation activity by methanotrophic bacteria. The
effect of spesific nitrogen application such as nitrite, nitrate, and ammonium to
methane oxidation activity was investigated. Methanotrophic bacteria consortium was
cultivated in nitrate mineral salts medium (NMS), treatment with 0.05% and 0,1%
concentration of specific nitrogen (nitrite, nitrate, and ammonium). Specific nitrogen
source application takes as inhibition and stimulation factor. The application of
nitrogen source can stimulate methane oxidation, with the highest methane oxidation
rate on 0.1% concentration of ammonium application (SN.NH4.0,01) was 8.619
ppm/L-1.m-1. Nitrogen source application also can inhibit methane oxidation, with the
lowest methane oxidation rate on 0,1% concentration of nitrate application was 3.925
ppm/L-1.m-1. High and low of utilization nitrogen source in system based on type and
concentration of nitrogen source application. The highest utilization of nitrogen
source was on ammonium application with 0.1% concentration.
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