Sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting HCV-RNA from chronic renal disease patient
Hepatitis C is a never-ending public health problem and the prevalence among chronic renal disease (CRD) patient always exceeds the general population. We studied the sensitivity of semi-nested reverse-transcription polymerase chain reaction (sn RT-PCR) technique for the detection of hepatitis C vir...
| Main Authors: | , , |
|---|---|
| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2011
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/13317/2/IHCI_2011_sensitivity_assay.pdf |
| _version_ | 1796876057493635072 |
|---|---|
| author | Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah , Nasuruddin |
| author_facet | Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah , Nasuruddin |
| author_sort | Hamzah, Hairul Aini |
| collection | IIUM |
| description | Hepatitis C is a never-ending public health problem and the prevalence among chronic renal disease (CRD) patient always exceeds the general population. We studied the sensitivity of semi-nested reverse-transcription polymerase chain reaction (sn RT-PCR) technique for the detection of hepatitis C virus (HCV) infection among patients with chronic renal problem who underwent haemodialysis treatment. An assay was evaluated using a positive control with known HCV viral load (2,148,000 IU/ml), which was determined at Gribbles Laboratory (Australia). A 10-fold serial dilution of the serum sample was conducted prior to RNA extraction and sn RT-PCR. The amplicon band was observed by electrophoresis in 1.7 % gel. Then, fifty-three (53) patients with known no infection to the HCV were collected and further tested with the established assay. The sensitivity result showed that the assay was sensitive enough to detect viral RNA up to 1:10 000 dilution (2.148 IU/ml). Further investigation on the serum samples of the CRD patients revealed four positive cases (7.6 %) which one of them seroconverted to positive. In conclusion, the established assay was highly sensitive in detecting HCV infection among seronegative CRD patient. The assay can be further improved and used as a HCV screening tool. |
| first_indexed | 2024-03-05T22:47:06Z |
| format | Proceeding Paper |
| id | oai:generic.eprints.org:13317 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:47:06Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:133172017-06-15T00:48:04Z http://irep.iium.edu.my/13317/ Sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting HCV-RNA from chronic renal disease patient Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah , Nasuruddin QR Microbiology QR355 Virology Hepatitis C is a never-ending public health problem and the prevalence among chronic renal disease (CRD) patient always exceeds the general population. We studied the sensitivity of semi-nested reverse-transcription polymerase chain reaction (sn RT-PCR) technique for the detection of hepatitis C virus (HCV) infection among patients with chronic renal problem who underwent haemodialysis treatment. An assay was evaluated using a positive control with known HCV viral load (2,148,000 IU/ml), which was determined at Gribbles Laboratory (Australia). A 10-fold serial dilution of the serum sample was conducted prior to RNA extraction and sn RT-PCR. The amplicon band was observed by electrophoresis in 1.7 % gel. Then, fifty-three (53) patients with known no infection to the HCV were collected and further tested with the established assay. The sensitivity result showed that the assay was sensitive enough to detect viral RNA up to 1:10 000 dilution (2.148 IU/ml). Further investigation on the serum samples of the CRD patients revealed four positive cases (7.6 %) which one of them seroconverted to positive. In conclusion, the established assay was highly sensitive in detecting HCV infection among seronegative CRD patient. The assay can be further improved and used as a HCV screening tool. 2011-12-07 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/13317/2/IHCI_2011_sensitivity_assay.pdf Hamzah, Hairul Aini and Mustafa Mahmoud, Mohammed Imad Al-Deen and Abdullah , Nasuruddin (2011) Sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting HCV-RNA from chronic renal disease patient. In: International Health Conference IIUM (IHCI) 2011 , 7-8 Dec 2011, Vistana Hotel Kuantan. http://www.iium.edu.my/ihsci2011/ |
| spellingShingle | QR Microbiology QR355 Virology Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah , Nasuruddin Sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting HCV-RNA from chronic renal disease patient |
| title | Sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting HCV-RNA from chronic renal disease patient |
| title_full | Sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting HCV-RNA from chronic renal disease patient |
| title_fullStr | Sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting HCV-RNA from chronic renal disease patient |
| title_full_unstemmed | Sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting HCV-RNA from chronic renal disease patient |
| title_short | Sensitivity assay of semi-nested reverse-transcription polymerase chain reaction in detecting HCV-RNA from chronic renal disease patient |
| title_sort | sensitivity assay of semi nested reverse transcription polymerase chain reaction in detecting hcv rna from chronic renal disease patient |
| topic | QR Microbiology QR355 Virology |
| url | http://irep.iium.edu.my/13317/2/IHCI_2011_sensitivity_assay.pdf |
| work_keys_str_mv | AT hamzahhairulaini sensitivityassayofseminestedreversetranscriptionpolymerasechainreactionindetectinghcvrnafromchronicrenaldiseasepatient AT mustafamahmoudmohammedimadaldeen sensitivityassayofseminestedreversetranscriptionpolymerasechainreactionindetectinghcvrnafromchronicrenaldiseasepatient AT abdullahnasuruddin sensitivityassayofseminestedreversetranscriptionpolymerasechainreactionindetectinghcvrnafromchronicrenaldiseasepatient |