| Summary: | Background. The respiratory care equipments have been identified as the potential vehicles,
which cause major nosocomial infections if these are colonized by fungi or bacteria. Medical
oxygen are a dry gas which need to be humidified to prevent respiratory mucosal system
injury. Oxygen humidifiers humidified gas by directing a stream of gas underwater, as the
gas bubbles rise to the surface, evaporation increases the water vapor content within the
bubble. If the water inside the reservoir are contaminated by bacteria, the bacteria could be
inhaled by the patients. The aim of this study is to compare the rate of bacterial
contamination for aquadest in reusable oxygen humidifier after 1 and 2 days at Dr Sardjito
Hospital Central Operating Theatre recovery room for multi-patient use.
Methods. Type of study is a prospective cohort study on 94 samples reusable oxygen
humidifiers sterilized by autoclave, divided into two groups, group A were used for multipatients,
and group B were not used to patient. Samples were labeled with number and
checked for the color. At day 0, aquadest in the reservoir before exposed to any patient was
aspirated 10 cc in syringes. The syringes then labelled with the sample number and date, and
examined for bacterial culture. At day 1, aquadest in reservoir that had been exposed for 24
hours was aspirated 10 cc in syringes, then labelled with the sample number and date, and
examined for bacterial culture. The reservoir the emptied and refilled with sterile aquadest.
The procedures were repeated in day 2.
Results. At day-0, all of the culture from group A are negative, while from group B there is 1
positive sample. At day-1, there are 5 sample show positif result from 47 samples (10.6%) in
group A, while in the group B there are 2 positive result from 47 samples (4.2%). RR: 2.5 CI
95 CI
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