| Summary: | Johar or Cassia siamea Lamk. classified as plant which is easily grow in
tropical country like Indonesia. Barakol in johar leaves has anxiolytic effect for
sleeping disturbance. This condition can be used by traditional medicine business
person. The choice of extraction method is an important thing to obtain optimal
active compound. This research is carried for knowing extraction method which
resulted maximum content of barakol.
Johar leaves powder is extracted by macerating for 24 hours and infusing
for 30 minutes, until the concentrated extract obtained. Maceration extract (M) is
fractioned by n-hexane and infudation extract for 30 minutes, known decoct (D),
fractioned by ethanol 96%, so that n-hexane soluble fraction (FLH), n-hexane
insoluble fraction (FTLH), ethanol soluble fraction (FLE), and ethanol insoluble
fraction (FTLE) are obtained. Relative content of barakol is measured by TLCdensitometry
with isolated barakol as reference. Chloroform:ethanol (9:1) is used
as mobile phase and silica gel F254 is used as stationary phase. The barakol content
is analyzed with one way ANOVA in convidence level of 95%.
The difference in extraction method and fractionation effects extracted
barakol relative content. Effective extraction method which resulted highest
barakol relative content is maceration in ethanol 96% without fractionation.
|
|---|