PENGEMBANGAN DAN VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR CIPROFLOXACIN DALAM SPIKED PLASMA MANUSIA DAN APLIKASINYA UNTUK UJI BIOEKIVALENSI
Ciprofloxacin is a fluoroquinolone type antibiotic agent that is being widely used for the treatment of several bacterial infections. It exhibits broad spectrum antimicrobial activity against Gram negative and Gram positive bacteria. This study aims to develop and validate an HPLC method couple with...
| Main Authors: | , |
|---|---|
| Format: | Thesis |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2014
|
| Subjects: |
| _version_ | 1797036032406847488 |
|---|---|
| author | , Anggun Aji Mukti , Dr. rer.nat. RR. Endang Lukitaningsih, M.Si., Apt. |
| author_facet | , Anggun Aji Mukti , Dr. rer.nat. RR. Endang Lukitaningsih, M.Si., Apt. |
| author_sort | , Anggun Aji Mukti |
| collection | UGM |
| description | Ciprofloxacin is a fluoroquinolone type antibiotic agent that is being widely
used for the treatment of several bacterial infections. It exhibits broad spectrum
antimicrobial activity against Gram negative and Gram positive bacteria. This
study aims to develop and validate an HPLC method couple with a UV detection
for determination of ciprofloxacin in spiked human plasma and its application for
bioequivalence study.
HPLC method using an RP-C18 column (Lachrom Hitachi, 250 x 4,6 mm.,
5μm), mobile phase of phosphate buffer (pH 3 ± 0,1 ) : acetonitrile (87 : 13) , a
flow rate of 1,5 mL / min with ultraviolet detection at 276,6 nm. The limid of
detection is 0,07 μg/mL. The regression equation are linear (r > 0,999)
This method was successfully applied to bioequivalence study of two
product of 500 mg ciprofloxacin. For bioequivalence study was obtained (90%
CI) ratio of Cmax = 74,08-124,60%, (90% CI) AUCt(0-12) ratio = 83,53-122,14 %
and (90% CI) ratio AUCt(0-
) = 86,07- 120,92%. |
| first_indexed | 2024-03-13T23:39:44Z |
| format | Thesis |
| id | oai:generic.eprints.org:133584 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:39:44Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1335842016-03-04T07:53:04Z https://repository.ugm.ac.id/133584/ PENGEMBANGAN DAN VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR CIPROFLOXACIN DALAM SPIKED PLASMA MANUSIA DAN APLIKASINYA UNTUK UJI BIOEKIVALENSI , Anggun Aji Mukti , Dr. rer.nat. RR. Endang Lukitaningsih, M.Si., Apt. ETD Ciprofloxacin is a fluoroquinolone type antibiotic agent that is being widely used for the treatment of several bacterial infections. It exhibits broad spectrum antimicrobial activity against Gram negative and Gram positive bacteria. This study aims to develop and validate an HPLC method couple with a UV detection for determination of ciprofloxacin in spiked human plasma and its application for bioequivalence study. HPLC method using an RP-C18 column (Lachrom Hitachi, 250 x 4,6 mm., 5μm), mobile phase of phosphate buffer (pH 3 ± 0,1 ) : acetonitrile (87 : 13) , a flow rate of 1,5 mL / min with ultraviolet detection at 276,6 nm. The limid of detection is 0,07 μg/mL. The regression equation are linear (r > 0,999) This method was successfully applied to bioequivalence study of two product of 500 mg ciprofloxacin. For bioequivalence study was obtained (90% CI) ratio of Cmax = 74,08-124,60%, (90% CI) AUCt(0-12) ratio = 83,53-122,14 % and (90% CI) ratio AUCt(0- ) = 86,07- 120,92%. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , Anggun Aji Mukti and , Dr. rer.nat. RR. Endang Lukitaningsih, M.Si., Apt. (2014) PENGEMBANGAN DAN VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR CIPROFLOXACIN DALAM SPIKED PLASMA MANUSIA DAN APLIKASINYA UNTUK UJI BIOEKIVALENSI. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=74287 |
| spellingShingle | ETD , Anggun Aji Mukti , Dr. rer.nat. RR. Endang Lukitaningsih, M.Si., Apt. PENGEMBANGAN DAN VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR CIPROFLOXACIN DALAM SPIKED PLASMA MANUSIA DAN APLIKASINYA UNTUK UJI BIOEKIVALENSI |
| title | PENGEMBANGAN DAN VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR CIPROFLOXACIN DALAM SPIKED PLASMA MANUSIA DAN APLIKASINYA UNTUK UJI BIOEKIVALENSI |
| title_full | PENGEMBANGAN DAN VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR CIPROFLOXACIN DALAM SPIKED PLASMA MANUSIA DAN APLIKASINYA UNTUK UJI BIOEKIVALENSI |
| title_fullStr | PENGEMBANGAN DAN VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR CIPROFLOXACIN DALAM SPIKED PLASMA MANUSIA DAN APLIKASINYA UNTUK UJI BIOEKIVALENSI |
| title_full_unstemmed | PENGEMBANGAN DAN VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR CIPROFLOXACIN DALAM SPIKED PLASMA MANUSIA DAN APLIKASINYA UNTUK UJI BIOEKIVALENSI |
| title_short | PENGEMBANGAN DAN VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR CIPROFLOXACIN DALAM SPIKED PLASMA MANUSIA DAN APLIKASINYA UNTUK UJI BIOEKIVALENSI |
| title_sort | pengembangan dan validasi metode kromatografi cair kinerja tinggi untuk penetapan kadar ciprofloxacin dalam spiked plasma manusia dan aplikasinya untuk uji bioekivalensi |
| topic | ETD |
| work_keys_str_mv | AT anggunajimukti pengembangandanvalidasimetodekromatograficairkinerjatinggiuntukpenetapankadarciprofloxacindalamspikedplasmamanusiadanaplikasinyauntukujibioekivalensi AT drrernatrrendanglukitaningsihmsiapt pengembangandanvalidasimetodekromatograficairkinerjatinggiuntukpenetapankadarciprofloxacindalamspikedplasmamanusiadanaplikasinyauntukujibioekivalensi |