ISOLASI METABOLIT SEKUNDER DARI JINTEN HITAM LOKAL (Nigella sativa L) DAN UJI AKTIVITAS ANTIOKSIDAN
This study was aimed to isolate secondary metabolites of the local black cumin seeds extract as quality indicator and determine antioxidant activity. Sample was obtained from local market, Pasar Gede, Solo which was origanally planted in Salatiga. This samples did not produce...
| Main Authors: | , |
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| Format: | Thesis |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| Subjects: |
| _version_ | 1797036056202182656 |
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| author | , ANDI SUHENDI , Dr. rer. nat. Nurul Hidayat A, M.Si. |
| author_facet | , ANDI SUHENDI , Dr. rer. nat. Nurul Hidayat A, M.Si. |
| author_sort | , ANDI SUHENDI |
| collection | UGM |
| description | This study was aimed to isolate secondary metabolites of the local black
cumin seeds extract as quality indicator and determine antioxidant activity.
Sample was obtained from local market, Pasar Gede, Solo which was origanally
planted in Salatiga. This samples did not produce essential oils if it was distilled
nwhile samples from Habasyah, Iran, India and Tunisia produced essential oils.
Active compound from black cumin is thymoquinon which was identified using
NMR and GCMS towards hexane and ethanol fractions. The antioxidant activities
of fractions of hexane and ethanol extracts of black cumin were performed by
DPPH (1, 1 � diphenyl-2-picryl hydrazyl) method. Compounds that have the
potential as chemical markers were isolated in the hexane fraction by radial
chromatography. Isolates were characterized by proton and carbon NMR and
mass spectrometry.
The antioxidant activity of ethanol extracts had IC50
value better than
methanol extracts, but this result has not satisfied yet. To confirm the antioxidant
activity, hexane and ethanol fractions of ethanol extract were asssessed. The
results of hexane and ethanol fraction possesed IC
50
value of 400.55 and 835.88
ppm, respectively. Thymoquinon did not identified by NMR and GCMS. Based
on the spectra of
1
H and
13
C-NMR, the major components from hexane fraction
were fatty acids derivatives, while in the ethanol fraction were sugar derivatives.
Profiling results of hexane fraction extracts of black cumin with GCMS showed
that the majority compounds were methyl ester octadeca-9,12-dienoic (32.03%
with similarity index (SI) = 97%) and ethyl linoleic (19.84% with SI = 92%),
while in the ethanol fraction were hexedecanoic acid methyl ester (31.33% with SI
= 97%) and 9-octadecanoic acid methyl ester (25.58% with SI = 97%). Isolate
obtained was strongly suspected linoleic acid based on data IR,
1
H and
13
C-NMR. |
| first_indexed | 2024-03-13T23:40:06Z |
| format | Thesis |
| id | oai:generic.eprints.org:133703 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:40:06Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1337032016-03-04T07:52:07Z https://repository.ugm.ac.id/133703/ ISOLASI METABOLIT SEKUNDER DARI JINTEN HITAM LOKAL (Nigella sativa L) DAN UJI AKTIVITAS ANTIOKSIDAN , ANDI SUHENDI , Dr. rer. nat. Nurul Hidayat A, M.Si. ETD This study was aimed to isolate secondary metabolites of the local black cumin seeds extract as quality indicator and determine antioxidant activity. Sample was obtained from local market, Pasar Gede, Solo which was origanally planted in Salatiga. This samples did not produce essential oils if it was distilled nwhile samples from Habasyah, Iran, India and Tunisia produced essential oils. Active compound from black cumin is thymoquinon which was identified using NMR and GCMS towards hexane and ethanol fractions. The antioxidant activities of fractions of hexane and ethanol extracts of black cumin were performed by DPPH (1, 1 � diphenyl-2-picryl hydrazyl) method. Compounds that have the potential as chemical markers were isolated in the hexane fraction by radial chromatography. Isolates were characterized by proton and carbon NMR and mass spectrometry. The antioxidant activity of ethanol extracts had IC50 value better than methanol extracts, but this result has not satisfied yet. To confirm the antioxidant activity, hexane and ethanol fractions of ethanol extract were asssessed. The results of hexane and ethanol fraction possesed IC 50 value of 400.55 and 835.88 ppm, respectively. Thymoquinon did not identified by NMR and GCMS. Based on the spectra of 1 H and 13 C-NMR, the major components from hexane fraction were fatty acids derivatives, while in the ethanol fraction were sugar derivatives. Profiling results of hexane fraction extracts of black cumin with GCMS showed that the majority compounds were methyl ester octadeca-9,12-dienoic (32.03% with similarity index (SI) = 97%) and ethyl linoleic (19.84% with SI = 92%), while in the ethanol fraction were hexedecanoic acid methyl ester (31.33% with SI = 97%) and 9-octadecanoic acid methyl ester (25.58% with SI = 97%). Isolate obtained was strongly suspected linoleic acid based on data IR, 1 H and 13 C-NMR. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , ANDI SUHENDI and , Dr. rer. nat. Nurul Hidayat A, M.Si. (2014) ISOLASI METABOLIT SEKUNDER DARI JINTEN HITAM LOKAL (Nigella sativa L) DAN UJI AKTIVITAS ANTIOKSIDAN. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=74478 |
| spellingShingle | ETD , ANDI SUHENDI , Dr. rer. nat. Nurul Hidayat A, M.Si. ISOLASI METABOLIT SEKUNDER DARI JINTEN HITAM LOKAL (Nigella sativa L) DAN UJI AKTIVITAS ANTIOKSIDAN |
| title | ISOLASI METABOLIT SEKUNDER DARI JINTEN HITAM LOKAL (Nigella sativa L) DAN UJI AKTIVITAS ANTIOKSIDAN |
| title_full | ISOLASI METABOLIT SEKUNDER DARI JINTEN HITAM LOKAL (Nigella sativa L) DAN UJI AKTIVITAS ANTIOKSIDAN |
| title_fullStr | ISOLASI METABOLIT SEKUNDER DARI JINTEN HITAM LOKAL (Nigella sativa L) DAN UJI AKTIVITAS ANTIOKSIDAN |
| title_full_unstemmed | ISOLASI METABOLIT SEKUNDER DARI JINTEN HITAM LOKAL (Nigella sativa L) DAN UJI AKTIVITAS ANTIOKSIDAN |
| title_short | ISOLASI METABOLIT SEKUNDER DARI JINTEN HITAM LOKAL (Nigella sativa L) DAN UJI AKTIVITAS ANTIOKSIDAN |
| title_sort | isolasi metabolit sekunder dari jinten hitam lokal nigella sativa l dan uji aktivitas antioksidan |
| topic | ETD |
| work_keys_str_mv | AT andisuhendi isolasimetabolitsekunderdarijintenhitamlokalnigellasativaldanujiaktivitasantioksidan AT drrernatnurulhidayatamsi isolasimetabolitsekunderdarijintenhitamlokalnigellasativaldanujiaktivitasantioksidan |