ISOLASI METABOLIT SEKUNDER DARI DAUN BANGUN-BANGUN (Coleus amboinicus Lour.) SERTA POTENSIIYYA SEBAGAI ANTIOKSIDAN
Isolation of the active compounds of secondary metabolites as antioxidant from bangun-bangun leaves, Identification and determine the potential activity of the active compound isolated from bangun-bangun leaves extract as an antioxidant determine the structure of the active...
| Main Authors: | , |
|---|---|
| Format: | Thesis |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| Subjects: |
| _version_ | 1797036115585138688 |
|---|---|
| author | , Kasta Gurning , Dr. Winarto Haryadi, M.Si. |
| author_facet | , Kasta Gurning , Dr. Winarto Haryadi, M.Si. |
| author_sort | , Kasta Gurning |
| collection | UGM |
| description | Isolation of the active compounds of secondary metabolites as antioxidant
from bangun-bangun leaves, Identification and determine the potential activity of
the active compound isolated from bangun-bangun leaves extract as an
antioxidant determine the structure of the active antioxidant compounds isolated
from bangun-bangun leaves with the analysis had been conducted by I& UV, GCMS,
rH-NM&
and
13C-NN{R.
The First, samples of leaves bangun-bangun were prepared and dried in
the open space by avoiding direct exposure to sunlight and the sample size by
using a blender. The Secon{ maceration process using methanol solvent. The
third, the extract was filtered, concentrated by an evaporator, a partition based on
the increase in the polarity of solvent (z-hexane, chlorofom, and ethyl acetate),
phyochemical test, and preliminary testing of each antioxidant extracts with
DPPH. The fourttl extracts with higher activity was purified by column
chromatography. The fifth, active isolates (crystal) were analyzed for melting
point, U/, IR\" GC-MS,
'H-NN,R.
and
I3C-MUR.
Isolates obtained results column two crystals with a melting point of
114-115
oC
and 232-233
oC,
crystal was obtained dissolve completely with
methanol, ethyl acetate and chloroform. The results of GC-MS analysis of the
active isolates I, there is a retention time peak with 7.316 minutes and percent
peak 100%. Active isolates II, there are two peaks, the first retention time of 6.658
minutes with percent peak3.95Yo. the socond has retention time of 9.001 minutes
with percent pak 96.A5%. Each crystal has antioxidant activity
flCso)
of 388.40
and 338.15 including antioxidant active compounds into the weak category. The
first active isolates were 7o-acetoxyroyleanone-16-hidroxy, and isolates the active
second were 7u-hidroxyroyleanone-l 6-acetoxy. |
| first_indexed | 2024-03-13T23:41:07Z |
| format | Thesis |
| id | oai:generic.eprints.org:133983 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:41:07Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1339832016-03-04T07:58:07Z https://repository.ugm.ac.id/133983/ ISOLASI METABOLIT SEKUNDER DARI DAUN BANGUN-BANGUN (Coleus amboinicus Lour.) SERTA POTENSIIYYA SEBAGAI ANTIOKSIDAN , Kasta Gurning , Dr. Winarto Haryadi, M.Si. ETD Isolation of the active compounds of secondary metabolites as antioxidant from bangun-bangun leaves, Identification and determine the potential activity of the active compound isolated from bangun-bangun leaves extract as an antioxidant determine the structure of the active antioxidant compounds isolated from bangun-bangun leaves with the analysis had been conducted by I& UV, GCMS, rH-NM& and 13C-NN{R. The First, samples of leaves bangun-bangun were prepared and dried in the open space by avoiding direct exposure to sunlight and the sample size by using a blender. The Secon{ maceration process using methanol solvent. The third, the extract was filtered, concentrated by an evaporator, a partition based on the increase in the polarity of solvent (z-hexane, chlorofom, and ethyl acetate), phyochemical test, and preliminary testing of each antioxidant extracts with DPPH. The fourttl extracts with higher activity was purified by column chromatography. The fifth, active isolates (crystal) were analyzed for melting point, U/, IR\" GC-MS, 'H-NN,R. and I3C-MUR. Isolates obtained results column two crystals with a melting point of 114-115 oC and 232-233 oC, crystal was obtained dissolve completely with methanol, ethyl acetate and chloroform. The results of GC-MS analysis of the active isolates I, there is a retention time peak with 7.316 minutes and percent peak 100%. Active isolates II, there are two peaks, the first retention time of 6.658 minutes with percent peak3.95Yo. the socond has retention time of 9.001 minutes with percent pak 96.A5%. Each crystal has antioxidant activity flCso) of 388.40 and 338.15 including antioxidant active compounds into the weak category. The first active isolates were 7o-acetoxyroyleanone-16-hidroxy, and isolates the active second were 7u-hidroxyroyleanone-l 6-acetoxy. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , Kasta Gurning and , Dr. Winarto Haryadi, M.Si. (2014) ISOLASI METABOLIT SEKUNDER DARI DAUN BANGUN-BANGUN (Coleus amboinicus Lour.) SERTA POTENSIIYYA SEBAGAI ANTIOKSIDAN. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=74917 |
| spellingShingle | ETD , Kasta Gurning , Dr. Winarto Haryadi, M.Si. ISOLASI METABOLIT SEKUNDER DARI DAUN BANGUN-BANGUN (Coleus amboinicus Lour.) SERTA POTENSIIYYA SEBAGAI ANTIOKSIDAN |
| title | ISOLASI METABOLIT SEKUNDER DARI DAUN BANGUN-BANGUN (Coleus amboinicus Lour.) SERTA POTENSIIYYA SEBAGAI ANTIOKSIDAN |
| title_full | ISOLASI METABOLIT SEKUNDER DARI DAUN BANGUN-BANGUN (Coleus amboinicus Lour.) SERTA POTENSIIYYA SEBAGAI ANTIOKSIDAN |
| title_fullStr | ISOLASI METABOLIT SEKUNDER DARI DAUN BANGUN-BANGUN (Coleus amboinicus Lour.) SERTA POTENSIIYYA SEBAGAI ANTIOKSIDAN |
| title_full_unstemmed | ISOLASI METABOLIT SEKUNDER DARI DAUN BANGUN-BANGUN (Coleus amboinicus Lour.) SERTA POTENSIIYYA SEBAGAI ANTIOKSIDAN |
| title_short | ISOLASI METABOLIT SEKUNDER DARI DAUN BANGUN-BANGUN (Coleus amboinicus Lour.) SERTA POTENSIIYYA SEBAGAI ANTIOKSIDAN |
| title_sort | isolasi metabolit sekunder dari daun bangun bangun coleus amboinicus lour serta potensiiyya sebagai antioksidan |
| topic | ETD |
| work_keys_str_mv | AT kastagurning isolasimetabolitsekunderdaridaunbangunbanguncoleusamboinicusloursertapotensiiyyasebagaiantioksidan AT drwinartoharyadimsi isolasimetabolitsekunderdaridaunbangunbanguncoleusamboinicusloursertapotensiiyyasebagaiantioksidan |