SENSITIVITAS RAPID DIAGNOSTIC TEST UNTUK SKRINING MALARIA PADA DARAH DONOR
Background: Malaria is one of the most important transfusion associated infections in many parts of the world, particularly the developing countries where it is endemic, including Indonesia. Blood is routinely screened for transfusion transmissible illnesses but not malaria. Screening for transfusio...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| author | , dr. Dwi Kurniawan Nugroho , Prof. dr. Supargiyono, DTM. |
| author_facet | , dr. Dwi Kurniawan Nugroho , Prof. dr. Supargiyono, DTM. |
| author_sort | , dr. Dwi Kurniawan Nugroho |
| collection | UGM |
| description | Background: Malaria is one of the most important transfusion associated infections in
many parts of the world, particularly the developing countries where it is endemic,
including Indonesia. Blood is routinely screened for transfusion transmissible illnesses
but not malaria. Screening for transfusion transmitted malaria to exclude blood
donations at risk of transmitting infection from donors to recipients is a critical part of
the process of ensuring that transfusion is as safe as possible. Direct detection of
parasite by microscopic examination is still a gold standard. However, the technique is
time-consuming, highly operator-dependent and prone to error. Consequently there is a
risk that this approach will not detect lower levels of parasitaemia where transmission
may still occur. Malaria rapid diagnostic tests (RDT), in simple kit form, can provide
results based on fingerprick or venous blood within minutes and may be better able to
identify parasitaemic donations, including those with much lower levels of parasites
than are reliably detectable by blood smear. The validity of RDT is various according to
antigen target and the trade mark.
Objectives: To evaluate the sensitivity of the malaria rapid diagnostic test in
comparison with microscopic blood smear for malaria blood donors screening,
especially in an endemic area.
Methods: A diagnostic test is carried out in the study, an alternative test is compared by
a microscopic conventional method as a gold standard. Research is done in Maumere,
Sikka District, East Nusa Tenggara. Subject involved in the study are potential blood
donors in the Blood Transfusion Unit, TC Hillers Maumere. Venous blood of donors
was screened for malaria parasites using rapid diagnostic test and Giemsa-stained and
the films examined under the microscope for malaria parasite. Parasite densities were
estimated for the malaria positive slides. The result analyzed by descriptive statistic test
and 2x2 table comparison test method.
Results: Diagnostic test data analysis showed that the rapid diagnostic test revealed
97,4% sensitivity, 100% spesificity, 100% positive predictive value, and 99,8%
negative predictive value.
Conclusions: It can be concluded that rapid diagnostic test is reliable to be used as a
malaria screening for blood donors in endemic area. |
| first_indexed | 2024-03-13T23:41:18Z |
| format | Thesis |
| id | oai:generic.eprints.org:134045 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:41:18Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1340452016-03-04T08:19:31Z https://repository.ugm.ac.id/134045/ SENSITIVITAS RAPID DIAGNOSTIC TEST UNTUK SKRINING MALARIA PADA DARAH DONOR , dr. Dwi Kurniawan Nugroho , Prof. dr. Supargiyono, DTM. ETD Background: Malaria is one of the most important transfusion associated infections in many parts of the world, particularly the developing countries where it is endemic, including Indonesia. Blood is routinely screened for transfusion transmissible illnesses but not malaria. Screening for transfusion transmitted malaria to exclude blood donations at risk of transmitting infection from donors to recipients is a critical part of the process of ensuring that transfusion is as safe as possible. Direct detection of parasite by microscopic examination is still a gold standard. However, the technique is time-consuming, highly operator-dependent and prone to error. Consequently there is a risk that this approach will not detect lower levels of parasitaemia where transmission may still occur. Malaria rapid diagnostic tests (RDT), in simple kit form, can provide results based on fingerprick or venous blood within minutes and may be better able to identify parasitaemic donations, including those with much lower levels of parasites than are reliably detectable by blood smear. The validity of RDT is various according to antigen target and the trade mark. Objectives: To evaluate the sensitivity of the malaria rapid diagnostic test in comparison with microscopic blood smear for malaria blood donors screening, especially in an endemic area. Methods: A diagnostic test is carried out in the study, an alternative test is compared by a microscopic conventional method as a gold standard. Research is done in Maumere, Sikka District, East Nusa Tenggara. Subject involved in the study are potential blood donors in the Blood Transfusion Unit, TC Hillers Maumere. Venous blood of donors was screened for malaria parasites using rapid diagnostic test and Giemsa-stained and the films examined under the microscope for malaria parasite. Parasite densities were estimated for the malaria positive slides. The result analyzed by descriptive statistic test and 2x2 table comparison test method. Results: Diagnostic test data analysis showed that the rapid diagnostic test revealed 97,4% sensitivity, 100% spesificity, 100% positive predictive value, and 99,8% negative predictive value. Conclusions: It can be concluded that rapid diagnostic test is reliable to be used as a malaria screening for blood donors in endemic area. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , dr. Dwi Kurniawan Nugroho and , Prof. dr. Supargiyono, DTM. (2014) SENSITIVITAS RAPID DIAGNOSTIC TEST UNTUK SKRINING MALARIA PADA DARAH DONOR. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=75018 |
| spellingShingle | ETD , dr. Dwi Kurniawan Nugroho , Prof. dr. Supargiyono, DTM. SENSITIVITAS RAPID DIAGNOSTIC TEST UNTUK SKRINING MALARIA PADA DARAH DONOR |
| title | SENSITIVITAS RAPID DIAGNOSTIC TEST UNTUK SKRINING MALARIA PADA DARAH DONOR |
| title_full | SENSITIVITAS RAPID DIAGNOSTIC TEST UNTUK SKRINING MALARIA PADA DARAH DONOR |
| title_fullStr | SENSITIVITAS RAPID DIAGNOSTIC TEST UNTUK SKRINING MALARIA PADA DARAH DONOR |
| title_full_unstemmed | SENSITIVITAS RAPID DIAGNOSTIC TEST UNTUK SKRINING MALARIA PADA DARAH DONOR |
| title_short | SENSITIVITAS RAPID DIAGNOSTIC TEST UNTUK SKRINING MALARIA PADA DARAH DONOR |
| title_sort | sensitivitas rapid diagnostic test untuk skrining malaria pada darah donor |
| topic | ETD |
| work_keys_str_mv | AT drdwikurniawannugroho sensitivitasrapiddiagnostictestuntukskriningmalariapadadarahdonor AT profdrsupargiyonodtm sensitivitasrapiddiagnostictestuntukskriningmalariapadadarahdonor |