PENGEMBANGAN LATERAL FLOW IMMUNOASSAY SEBAGAI ALAT DETEKSI AMPLIFIKASI ISOTERMAL VIRUS DENGUE
Dengue hemorrhagic fever (DHF) is a major disease in Indonesia. Early detection of the disease is expected to reduce the problems caused by the Dengue disease. Detection of dengue virus conducted by isothermal nucleic acid sequencebased amplification (NASBA) resulted in the elimination of thermocycl...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| author | , Yusnita M. Anggraeni , Dr. drh. Asmarani Kusumawati, MP. |
| author_facet | , Yusnita M. Anggraeni , Dr. drh. Asmarani Kusumawati, MP. |
| author_sort | , Yusnita M. Anggraeni |
| collection | UGM |
| description | Dengue hemorrhagic fever (DHF) is a major disease in Indonesia. Early
detection of the disease is expected to reduce the problems caused by the Dengue
disease. Detection of dengue virus conducted by isothermal nucleic acid sequencebased
amplification (NASBA) resulted in the elimination of thermocycler. Amplicon
of NASBA are detected by lateral flow immunoassay (LFIA) which is a simple
method.
This study was conducted to determine the NASBA primers� and LFIA probe�s
ability to detect Dengue virus, to determine the ability of LFIA with designed probe
compared to NASBA and RT-PCR, to measure the sensitivity and specificity of LFIA
in laboratory scale. The study began with the primers design for NASBA. Primers
design for RT-PCR performed to test whether the primers can run on NASBA
reaction. The NASBA reaction was performed on blood samples with positive
controls from C6/36 cell culture. Sensitivity assay of NASBA was performed by
dilution of Dengue virus serotype 3, followed by specificity assay using Chikungunya
virus. Product of NASBA was visualized on LFIA and agarose gel for comparison.
Probes construction for LFIA method can be used for Dengue virus detection,
indicated by the appearance of bands at the size of 200 bp. This method has a
sensitivity of up to 1 pg/ μl, lower than RT-PCR (0,1 pg/ μl). This method shows no
cross-reaction to Chikungunya virus. The results can be applied as a simple and
economical method for detection of Dengue virus. |
| first_indexed | 2024-03-13T23:41:28Z |
| format | Thesis |
| id | oai:generic.eprints.org:134099 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:41:28Z |
| publishDate | 2014 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1340992016-03-04T08:19:07Z https://repository.ugm.ac.id/134099/ PENGEMBANGAN LATERAL FLOW IMMUNOASSAY SEBAGAI ALAT DETEKSI AMPLIFIKASI ISOTERMAL VIRUS DENGUE , Yusnita M. Anggraeni , Dr. drh. Asmarani Kusumawati, MP. ETD Dengue hemorrhagic fever (DHF) is a major disease in Indonesia. Early detection of the disease is expected to reduce the problems caused by the Dengue disease. Detection of dengue virus conducted by isothermal nucleic acid sequencebased amplification (NASBA) resulted in the elimination of thermocycler. Amplicon of NASBA are detected by lateral flow immunoassay (LFIA) which is a simple method. This study was conducted to determine the NASBA primers� and LFIA probe�s ability to detect Dengue virus, to determine the ability of LFIA with designed probe compared to NASBA and RT-PCR, to measure the sensitivity and specificity of LFIA in laboratory scale. The study began with the primers design for NASBA. Primers design for RT-PCR performed to test whether the primers can run on NASBA reaction. The NASBA reaction was performed on blood samples with positive controls from C6/36 cell culture. Sensitivity assay of NASBA was performed by dilution of Dengue virus serotype 3, followed by specificity assay using Chikungunya virus. Product of NASBA was visualized on LFIA and agarose gel for comparison. Probes construction for LFIA method can be used for Dengue virus detection, indicated by the appearance of bands at the size of 200 bp. This method has a sensitivity of up to 1 pg/ μl, lower than RT-PCR (0,1 pg/ μl). This method shows no cross-reaction to Chikungunya virus. The results can be applied as a simple and economical method for detection of Dengue virus. [Yogyakarta] : Universitas Gadjah Mada 2014 Thesis NonPeerReviewed , Yusnita M. Anggraeni and , Dr. drh. Asmarani Kusumawati, MP. (2014) PENGEMBANGAN LATERAL FLOW IMMUNOASSAY SEBAGAI ALAT DETEKSI AMPLIFIKASI ISOTERMAL VIRUS DENGUE. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=75088 |
| spellingShingle | ETD , Yusnita M. Anggraeni , Dr. drh. Asmarani Kusumawati, MP. PENGEMBANGAN LATERAL FLOW IMMUNOASSAY SEBAGAI ALAT DETEKSI AMPLIFIKASI ISOTERMAL VIRUS DENGUE |
| title | PENGEMBANGAN LATERAL FLOW IMMUNOASSAY SEBAGAI ALAT DETEKSI AMPLIFIKASI ISOTERMAL VIRUS DENGUE |
| title_full | PENGEMBANGAN LATERAL FLOW IMMUNOASSAY SEBAGAI ALAT DETEKSI AMPLIFIKASI ISOTERMAL VIRUS DENGUE |
| title_fullStr | PENGEMBANGAN LATERAL FLOW IMMUNOASSAY SEBAGAI ALAT DETEKSI AMPLIFIKASI ISOTERMAL VIRUS DENGUE |
| title_full_unstemmed | PENGEMBANGAN LATERAL FLOW IMMUNOASSAY SEBAGAI ALAT DETEKSI AMPLIFIKASI ISOTERMAL VIRUS DENGUE |
| title_short | PENGEMBANGAN LATERAL FLOW IMMUNOASSAY SEBAGAI ALAT DETEKSI AMPLIFIKASI ISOTERMAL VIRUS DENGUE |
| title_sort | pengembangan lateral flow immunoassay sebagai alat deteksi amplifikasi isotermal virus dengue |
| topic | ETD |
| work_keys_str_mv | AT yusnitamanggraeni pengembanganlateralflowimmunoassaysebagaialatdeteksiamplifikasiisotermalvirusdengue AT drdrhasmaranikusumawatimp pengembanganlateralflowimmunoassaysebagaialatdeteksiamplifikasiisotermalvirusdengue |