| Summary: | The study was done to investigate the potency of angiotensin converting enzyme (ACE) inhibitor derived from protein hydrolysate, and to identify the potent ACE inhibitory peptide of Indonesian Local animals. The study used Bali cattle, Kacang goat, Native chicken, and Local duck meat. The study consisted of two stages. First, to investigate the potency of ACE inhibitory activity of protein hydrolysate of Bali cattle, Kacang goat, Native chicken, and Local duck meat. Meat extract was made by grinding and adding water to respective meat with meat to water ratio of 1:2. Meat extract was then tested on water soluble protein and ACE inhibitory activity. Meat extract was then hydrolyzed by pepsin at pH 2 and temperature of 37.5oC for 2 hours, and was continued by typsin and chymotrypsin at pH 7 and temperature of 37.5oC for 2 hours. Hydrolizate of meat protein was then filtered using Advanced Dismic-25ES filter. Filtrate of protein hydrolysate was then tested on water soluble protein and ACE inhibitory activity. The protein profile of meat extract and protein hydrolysate were also identified by SDS-PAGE.The second stage was done to identify the highest potent ACE inhibitory peptide. ACE inhibitory peptide of Kacang goat was purified by reverse phase high performance liquid chromatography (RP-HPLC). The results of the first stage showed that water soluble protein concentration of protein hydrolysate was higher than meat extract. The water soluble protein concentration of meat extract of Bali cattle, Kacang goat, Native chicken and Local duck were 14.00
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