| Summary: | Andrographis paniculata containing an active compound andrographolide,
and Curcuma xanthorrhiza containing curcumin, were reported to have potent
hypoglycemic activity. The aim of this study is to evaluate the antidiabetic effects
of the combination of andrographolide and curcumin in insulin resistance rats.
Andrographolide was isolated using gradual fractionation method.
Qualitative analysis of andrographolide used 2-dimensional TLC, whereas
quantitative analysis used HPLC. Insulin resistance in rat was induced by oral
administration of fructose 1.8 g/kgBW, 2 mL duck egg yolks, and 2 mL lard oil
for 120 consecutive days. Insulin resistance in rat was confirmed by using
hypoglycemic activity of glibenclamide on day 120. On the day of 120-134, rats
were administered with i.e, metformin 45 mg/kgBW as a positive control,
andrographolide 4.5 mg/kgBW, curcumin 45 mg/kgBW, combination
andrographolide:curcumin 1:3
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