KARAKTERISASI DAN IMOBILISASI PAPAIN GETAH BUAH PEPAYA GUNUNG: Carica pubescens Lanne & Koch
Supplying of papain in Indonesia still depends on import. Papain from mountain papaya (C. pubescens) fruit is potentially produced and utilized. Mountain papaya fruit has sour taste and plenty latex content, however it has not been used as source of papain. Information related to the characteristics...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2015
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| author | Andarias, S. HAFIDHAWATI |
| author_facet | Andarias, S. HAFIDHAWATI |
| author_sort | Andarias, S. HAFIDHAWATI |
| collection | UGM |
| description | Supplying of papain in Indonesia still depends on import. Papain from mountain papaya (C. pubescens) fruit is potentially produced and utilized. Mountain papaya fruit has sour taste and plenty latex content, however it has not been used as source of papain. Information related to the characteristics of papain of C. pubescens is limited. This research was aimed to determine the characteristics of papain of C. pubescens (optimum pH and temperature, molecular weight) as well as the activity of the immobilized papain. Latex obtained from the papaya plantations in Dieng Plateau, Central Java. The latex was tapped from 2-4 months old fruit. Purification of papain was done using ammonium sulphate and acetone precipitation. Papain activity was assayed by spectrophotometry using 1% casein as a substrate. The optimum pH and temperature of papain activity were determined with various pH and temperature. SDS-PAGE was used for determination of papain molecular weight. Papain is immobilized with sodium alginate as a matrix. Results of this study showed that the optimum activity at pH 7 and 60ºC of free papain is 5.763 U/mL, while the optimum activity of papain immobilized at pH 8 and 70ºC is 3.083 U/mL. The molecular weight of papain from C. pubescens is predicted arround 21.44 kDa approximately. The immobilized papain can be used only for three times repeatedly. |
| first_indexed | 2024-03-13T23:43:33Z |
| format | Thesis |
| id | oai:generic.eprints.org:134774 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T23:43:33Z |
| publishDate | 2015 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1347742016-04-14T07:27:28Z https://repository.ugm.ac.id/134774/ KARAKTERISASI DAN IMOBILISASI PAPAIN GETAH BUAH PEPAYA GUNUNG: Carica pubescens Lanne & Koch Andarias, S. HAFIDHAWATI Plant Biology Plant Developmental and Reproductive Biology Biological Sciences Supplying of papain in Indonesia still depends on import. Papain from mountain papaya (C. pubescens) fruit is potentially produced and utilized. Mountain papaya fruit has sour taste and plenty latex content, however it has not been used as source of papain. Information related to the characteristics of papain of C. pubescens is limited. This research was aimed to determine the characteristics of papain of C. pubescens (optimum pH and temperature, molecular weight) as well as the activity of the immobilized papain. Latex obtained from the papaya plantations in Dieng Plateau, Central Java. The latex was tapped from 2-4 months old fruit. Purification of papain was done using ammonium sulphate and acetone precipitation. Papain activity was assayed by spectrophotometry using 1% casein as a substrate. The optimum pH and temperature of papain activity were determined with various pH and temperature. SDS-PAGE was used for determination of papain molecular weight. Papain is immobilized with sodium alginate as a matrix. Results of this study showed that the optimum activity at pH 7 and 60ºC of free papain is 5.763 U/mL, while the optimum activity of papain immobilized at pH 8 and 70ºC is 3.083 U/mL. The molecular weight of papain from C. pubescens is predicted arround 21.44 kDa approximately. The immobilized papain can be used only for three times repeatedly. [Yogyakarta] : Universitas Gadjah Mada 2015 Thesis NonPeerReviewed Andarias, S. HAFIDHAWATI (2015) KARAKTERISASI DAN IMOBILISASI PAPAIN GETAH BUAH PEPAYA GUNUNG: Carica pubescens Lanne & Koch. Masters thesis, UGM. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=77908 |
| spellingShingle | Plant Biology Plant Developmental and Reproductive Biology Biological Sciences Andarias, S. HAFIDHAWATI KARAKTERISASI DAN IMOBILISASI PAPAIN GETAH BUAH PEPAYA GUNUNG: Carica pubescens Lanne & Koch |
| title | KARAKTERISASI DAN IMOBILISASI PAPAIN GETAH BUAH PEPAYA GUNUNG: Carica pubescens Lanne & Koch |
| title_full | KARAKTERISASI DAN IMOBILISASI PAPAIN GETAH BUAH PEPAYA GUNUNG: Carica pubescens Lanne & Koch |
| title_fullStr | KARAKTERISASI DAN IMOBILISASI PAPAIN GETAH BUAH PEPAYA GUNUNG: Carica pubescens Lanne & Koch |
| title_full_unstemmed | KARAKTERISASI DAN IMOBILISASI PAPAIN GETAH BUAH PEPAYA GUNUNG: Carica pubescens Lanne & Koch |
| title_short | KARAKTERISASI DAN IMOBILISASI PAPAIN GETAH BUAH PEPAYA GUNUNG: Carica pubescens Lanne & Koch |
| title_sort | karakterisasi dan imobilisasi papain getah buah pepaya gunung carica pubescens lanne koch |
| topic | Plant Biology Plant Developmental and Reproductive Biology Biological Sciences |
| work_keys_str_mv | AT andariasshafidhawati karakterisasidanimobilisasipapaingetahbuahpepayagunungcaricapubescenslannekoch |