| Summary: | The study aimed to determine the accuracy of ovarian follicle and corpus luteum measurements in vivo using
ultrasonography diagnostic and in vitro after slicing of the ovaries. A total of 15 cows were examined using
transrectal ultrasonography just before being slaughtered. Ultrasonographic examination was performed using
7.5 MHz transrectal tranducer on ovaries, measured dominant follicle and corpus luteum diameters. Follicle
appeared echogenic, black with round shape. Corpus luteum, appeared as low echogenicity structure and grayish
in color.Ovaries were taken out soon after animals slaughtered, soaked in 10% buffered formalin for 12 hours,
then cut in thin slices and measured follicle and corpus luteum diameters. Results of follicle and corpus luteum
diameter measurements in vitro and in vivo compared using two sample or paired t-test, correlation and
regression analyses. The two methods of follicle and corpus luteum measurements had linear correlation (follicle
measurement, R2 = 0.933 , r = 0.966, n = 15, P < 0.05) (corpus luteum, R2 = 0.912, r = 0.955, n = 15, P < 0.05).
Measurements of dominant follicle diameter and corpus luteum size manifested the real size of the two ovarian
structures. Transrectal ultrasonography diagnostic on ovaries proved to be having high accuracy and reliability
for identification, measurement of ovarian structure diameters, follicle and corpus luteum in cattle.
Key words: ultrasonography diagnostic, ovary, dominant follicle, corpus luteum, cattle
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