Study of Skin Premature Aging Induced by Ultraviolet B Light Irradiation in Balb/C Mouse Model
The aim of this study was to determine a process of skin premature aging in mouse model using Ultraviolet B light irradiation. Observed variable was structural changes of skin epidermis, collagen, and elastin by histochemistry staining. Twenty five and each group consisted of five, female B...
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Format: | Article |
Language: | English |
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Universitas Airlangga
2009
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Online Access: | https://repository.ugm.ac.id/134986/1/jurnal.pdf |
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author | Purwandari K., Yuli Widyarini, Sitarina |
author_facet | Purwandari K., Yuli Widyarini, Sitarina |
author_sort | Purwandari K., Yuli |
collection | UGM |
description | The aim of this study was to determine a process of skin premature aging in mouse model using Ultraviolet B light irradiation. Observed variable was structural changes of skin epidermis, collagen, and elastin by histochemistry staining. Twenty five and each group consisted of five, female Balb/c mice, four weeks old were experimental animal. The first group was as control ( unirradiated ), the second until fifth groups were given a UVB light irradiation during 5,10,15,20 weeks, respectively. The UVB dose was 465 mJ/m2 for 3 minutes. The hair of back mice was shaved before UVB light irradiation. The drinking water was given ad libitum. The observation was done macroscopical examination through histopatological changes of epidermis, collagen , and elastin fiber using H&E, Mallory and Alcian Blue staining. The results were analyzed by descriptive, Anova and Duncan Multiple Test. |
first_indexed | 2024-03-13T23:44:05Z |
format | Article |
id | oai:generic.eprints.org:134986 |
institution | Universiti Gadjah Mada |
language | English |
last_indexed | 2024-03-13T23:44:05Z |
publishDate | 2009 |
publisher | Universitas Airlangga |
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spelling | oai:generic.eprints.org:1349862015-06-01T06:35:58Z https://repository.ugm.ac.id/134986/ Study of Skin Premature Aging Induced by Ultraviolet B Light Irradiation in Balb/C Mouse Model Purwandari K., Yuli Widyarini, Sitarina Veterinary Sciences The aim of this study was to determine a process of skin premature aging in mouse model using Ultraviolet B light irradiation. Observed variable was structural changes of skin epidermis, collagen, and elastin by histochemistry staining. Twenty five and each group consisted of five, female Balb/c mice, four weeks old were experimental animal. The first group was as control ( unirradiated ), the second until fifth groups were given a UVB light irradiation during 5,10,15,20 weeks, respectively. The UVB dose was 465 mJ/m2 for 3 minutes. The hair of back mice was shaved before UVB light irradiation. The drinking water was given ad libitum. The observation was done macroscopical examination through histopatological changes of epidermis, collagen , and elastin fiber using H&E, Mallory and Alcian Blue staining. The results were analyzed by descriptive, Anova and Duncan Multiple Test. Universitas Airlangga 2009-05 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/134986/1/jurnal.pdf Purwandari K., Yuli and Widyarini, Sitarina (2009) Study of Skin Premature Aging Induced by Ultraviolet B Light Irradiation in Balb/C Mouse Model. Media Kedokteran Hewan, 25 (2). pp. 96-103. ISSN 0215-8930 http://journal.unair.ac.id/ |
spellingShingle | Veterinary Sciences Purwandari K., Yuli Widyarini, Sitarina Study of Skin Premature Aging Induced by Ultraviolet B Light Irradiation in Balb/C Mouse Model |
title | Study of Skin Premature Aging Induced by Ultraviolet B Light Irradiation in Balb/C Mouse Model |
title_full | Study of Skin Premature Aging Induced by Ultraviolet B Light Irradiation in Balb/C Mouse Model |
title_fullStr | Study of Skin Premature Aging Induced by Ultraviolet B Light Irradiation in Balb/C Mouse Model |
title_full_unstemmed | Study of Skin Premature Aging Induced by Ultraviolet B Light Irradiation in Balb/C Mouse Model |
title_short | Study of Skin Premature Aging Induced by Ultraviolet B Light Irradiation in Balb/C Mouse Model |
title_sort | study of skin premature aging induced by ultraviolet b light irradiation in balb c mouse model |
topic | Veterinary Sciences |
url | https://repository.ugm.ac.id/134986/1/jurnal.pdf |
work_keys_str_mv | AT purwandarikyuli studyofskinprematureaginginducedbyultravioletblightirradiationinbalbcmousemodel AT widyarinisitarina studyofskinprematureaginginducedbyultravioletblightirradiationinbalbcmousemodel |