Isolation and Identification of Lactic Acid Bacteria from Peranakan Etawa h Crossbred Goat Milk
Lactic acid bacteria (LAB) are widely used in dairy fermentation. The aim of this study was to isolate and identify LAB from fresh milk of Peranakan Etawah crossbred goat in Yogyakarta, Indonesia. Fresh milk sample from three different farms were collected after milking and plated in to de M...
| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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2014
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| Online Access: | https://repository.ugm.ac.id/135337/1/Isolation%20and%20Identification%20of%20Lactic%20Acid%20Bacteria%20from%20Peranakan%20Etawa%20h%20%20Crossbred%20Goat%20Milk.pdf |
| _version_ | 1826049361321132032 |
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| author | Widodo, Widodo Indratiningsih, Indratiningsih Nurliyani, Nurliyani Endang Wahyuni, Endang Wahyuni Tiyas Tono Taufiq, Tiyas Tono Taufiq |
| author_facet | Widodo, Widodo Indratiningsih, Indratiningsih Nurliyani, Nurliyani Endang Wahyuni, Endang Wahyuni Tiyas Tono Taufiq, Tiyas Tono Taufiq |
| author_sort | Widodo, Widodo |
| collection | UGM |
| description | Lactic acid bacteria (LAB) are widely used in dairy fermentation. The aim of this study was to isolate and identify LAB from fresh milk of Peranakan Etawah crossbred goat in Yogyakarta, Indonesia. Fresh milk sample from three different farms were collected after milking and plated in to de Mann Rogosa Sharp e (MRS) agar sup plemented with 0.5% ox bile. A single colony was purified with a streaking method, followed by morphology and
biochemical identification based on Gram staining, catalase test, motility, spore formation, and their growth on different carbon source s . Molecular identification was conducted based on the sequence of 16S rRNA gene. This study isolated four isolates from Peranakan Etawah
crossbred goat milk. Based on 16S rRNA gene sequences, isolate M101 and M102 showed homology with Lactococcus garvieae , isolate M103 showed homology with Pediococcus
pentosaceus , whereas isolate M104 showed homology with Lactobacillus paracasei subsp. Paracasei. Further experiments are being carried out to explore their ability and potential as starter culture for goat milk fermentation. Key Words: Goat milk, Lactic Acid Bacteria, Molecular identification. |
| first_indexed | 2024-03-13T23:44:45Z |
| format | Conference or Workshop Item |
| id | oai:generic.eprints.org:135337 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-13T23:44:45Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1353372015-11-06T08:24:28Z https://repository.ugm.ac.id/135337/ Isolation and Identification of Lactic Acid Bacteria from Peranakan Etawa h Crossbred Goat Milk Widodo, Widodo Indratiningsih, Indratiningsih Nurliyani, Nurliyani Endang Wahyuni, Endang Wahyuni Tiyas Tono Taufiq, Tiyas Tono Taufiq Agricultural Land Management Food technology Livestock Lactic acid bacteria (LAB) are widely used in dairy fermentation. The aim of this study was to isolate and identify LAB from fresh milk of Peranakan Etawah crossbred goat in Yogyakarta, Indonesia. Fresh milk sample from three different farms were collected after milking and plated in to de Mann Rogosa Sharp e (MRS) agar sup plemented with 0.5% ox bile. A single colony was purified with a streaking method, followed by morphology and biochemical identification based on Gram staining, catalase test, motility, spore formation, and their growth on different carbon source s . Molecular identification was conducted based on the sequence of 16S rRNA gene. This study isolated four isolates from Peranakan Etawah crossbred goat milk. Based on 16S rRNA gene sequences, isolate M101 and M102 showed homology with Lactococcus garvieae , isolate M103 showed homology with Pediococcus pentosaceus , whereas isolate M104 showed homology with Lactobacillus paracasei subsp. Paracasei. Further experiments are being carried out to explore their ability and potential as starter culture for goat milk fermentation. Key Words: Goat milk, Lactic Acid Bacteria, Molecular identification. 2014-11-10 Conference or Workshop Item PeerReviewed application/pdf en https://repository.ugm.ac.id/135337/1/Isolation%20and%20Identification%20of%20Lactic%20Acid%20Bacteria%20from%20Peranakan%20Etawa%20h%20%20Crossbred%20Goat%20Milk.pdf Widodo, Widodo and Indratiningsih, Indratiningsih and Nurliyani, Nurliyani and Endang Wahyuni, Endang Wahyuni and Tiyas Tono Taufiq, Tiyas Tono Taufiq (2014) Isolation and Identification of Lactic Acid Bacteria from Peranakan Etawa h Crossbred Goat Milk. In: The 16th AAAP Congress, 10-14 November 2014, Grha Sabha Pramana Universitas Gadjah Mada. |
| spellingShingle | Agricultural Land Management Food technology Livestock Widodo, Widodo Indratiningsih, Indratiningsih Nurliyani, Nurliyani Endang Wahyuni, Endang Wahyuni Tiyas Tono Taufiq, Tiyas Tono Taufiq Isolation and Identification of Lactic Acid Bacteria from Peranakan Etawa h Crossbred Goat Milk |
| title | Isolation and Identification of Lactic Acid Bacteria from Peranakan Etawa h Crossbred Goat Milk |
| title_full | Isolation and Identification of Lactic Acid Bacteria from Peranakan Etawa h Crossbred Goat Milk |
| title_fullStr | Isolation and Identification of Lactic Acid Bacteria from Peranakan Etawa h Crossbred Goat Milk |
| title_full_unstemmed | Isolation and Identification of Lactic Acid Bacteria from Peranakan Etawa h Crossbred Goat Milk |
| title_short | Isolation and Identification of Lactic Acid Bacteria from Peranakan Etawa h Crossbred Goat Milk |
| title_sort | isolation and identification of lactic acid bacteria from peranakan etawa h crossbred goat milk |
| topic | Agricultural Land Management Food technology Livestock |
| url | https://repository.ugm.ac.id/135337/1/Isolation%20and%20Identification%20of%20Lactic%20Acid%20Bacteria%20from%20Peranakan%20Etawa%20h%20%20Crossbred%20Goat%20Milk.pdf |
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