Efficient nursery production and multiple-shoot clumps formation from shoot tiller-derived shoot apices of dwarf napiergrass (Pennisetum purpureum Schumach.)
The objective of this study was to improve an in vitro tissue culture system for the dwarf napiergrass l.Pennisetum purpureum Schumach.). Additionally, it was needed special treatments for acclimatization, and carried the risks of somaclonal variation and physiological aberations. Shoot apices as...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Journal of Warm Regional Society of Animal Science, Japan
2012
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/135610/1/JOURNAL%20WARAS%202012.pdf |
| _version_ | 1826049405873029120 |
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| author | Nafiatul Umami, N. Umami Takahiro Gondo, T. Gondo Genki Ishigaki, G. Ishigaki Mohammad Mijanur Rahman, M. M. Rahman Ryo Akashi, R. Akashi |
| author_facet | Nafiatul Umami, N. Umami Takahiro Gondo, T. Gondo Genki Ishigaki, G. Ishigaki Mohammad Mijanur Rahman, M. M. Rahman Ryo Akashi, R. Akashi |
| author_sort | Nafiatul Umami, N. Umami |
| collection | UGM |
| description | The objective of this study was to improve an in vitro tissue culture system for the dwarf napiergrass
l.Pennisetum purpureum Schumach.). Additionally, it was needed special treatments for acclimatization, and carried the risks
of somaclonal variation and physiological aberations. Shoot apices as initial explants were isolated aseptically from shoottillers,
and cultured on in vitro MS (Murashige and Skoog, 1962) medium containing 3.002 sucrose and 0.3o/o phytagel. The
most effective phltohormone treatment for multiple-shoot clumps (MSCs) induction was 0.1 mg L-l 2,4-D plus 2.0 mg L-1
BAP. The addition of 50 p M CuSO+ could increase the percentage of MSCs proliferation. Plant regeneration frequency was
achieved up to 84%o by culturing the MSCs on solid MS medium containing 0.1 mg L-1 NAA and2.0 mg L-l BAP. A11
regenerants were successfully grown up in the soil. Compared to control plants, in vitro regefierated plants did not reveal any
significant difference (P > 0.05) on morphological characteristics and DNA content. The results of this study suggest that
improved protocols for in vitro propagation would provide high quality nursery plant production of dwarf napiergrass in
grassland field.
Key words: dwarf napiergrass; flow cytometry; multiple-shoot clumps formation; plant regeneration. |
| first_indexed | 2024-03-13T23:45:28Z |
| format | Article |
| id | oai:generic.eprints.org:135610 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-13T23:45:28Z |
| publishDate | 2012 |
| publisher | Journal of Warm Regional Society of Animal Science, Japan |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1356102016-01-20T02:30:48Z https://repository.ugm.ac.id/135610/ Efficient nursery production and multiple-shoot clumps formation from shoot tiller-derived shoot apices of dwarf napiergrass (Pennisetum purpureum Schumach.) Nafiatul Umami, N. Umami Takahiro Gondo, T. Gondo Genki Ishigaki, G. Ishigaki Mohammad Mijanur Rahman, M. M. Rahman Ryo Akashi, R. Akashi Agricultural Land Management Agronomy Husbandry The objective of this study was to improve an in vitro tissue culture system for the dwarf napiergrass l.Pennisetum purpureum Schumach.). Additionally, it was needed special treatments for acclimatization, and carried the risks of somaclonal variation and physiological aberations. Shoot apices as initial explants were isolated aseptically from shoottillers, and cultured on in vitro MS (Murashige and Skoog, 1962) medium containing 3.002 sucrose and 0.3o/o phytagel. The most effective phltohormone treatment for multiple-shoot clumps (MSCs) induction was 0.1 mg L-l 2,4-D plus 2.0 mg L-1 BAP. The addition of 50 p M CuSO+ could increase the percentage of MSCs proliferation. Plant regeneration frequency was achieved up to 84%o by culturing the MSCs on solid MS medium containing 0.1 mg L-1 NAA and2.0 mg L-l BAP. A11 regenerants were successfully grown up in the soil. Compared to control plants, in vitro regefierated plants did not reveal any significant difference (P > 0.05) on morphological characteristics and DNA content. The results of this study suggest that improved protocols for in vitro propagation would provide high quality nursery plant production of dwarf napiergrass in grassland field. Key words: dwarf napiergrass; flow cytometry; multiple-shoot clumps formation; plant regeneration. Journal of Warm Regional Society of Animal Science, Japan 2012 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/135610/1/JOURNAL%20WARAS%202012.pdf Nafiatul Umami, N. Umami and Takahiro Gondo, T. Gondo and Genki Ishigaki, G. Ishigaki and Mohammad Mijanur Rahman, M. M. Rahman and Ryo Akashi, R. Akashi (2012) Efficient nursery production and multiple-shoot clumps formation from shoot tiller-derived shoot apices of dwarf napiergrass (Pennisetum purpureum Schumach.). Journal of Warm Regional Society of Animal Science, Japan, 55 (2). pp. 121-127. ISSN 2185-081X http://waras.ac.affrc.go.jp/index.htm |
| spellingShingle | Agricultural Land Management Agronomy Husbandry Nafiatul Umami, N. Umami Takahiro Gondo, T. Gondo Genki Ishigaki, G. Ishigaki Mohammad Mijanur Rahman, M. M. Rahman Ryo Akashi, R. Akashi Efficient nursery production and multiple-shoot clumps formation from shoot tiller-derived shoot apices of dwarf napiergrass (Pennisetum purpureum Schumach.) |
| title | Efficient nursery production and multiple-shoot clumps formation from shoot tiller-derived shoot apices of dwarf napiergrass (Pennisetum purpureum Schumach.) |
| title_full | Efficient nursery production and multiple-shoot clumps formation from shoot tiller-derived shoot apices of dwarf napiergrass (Pennisetum purpureum Schumach.) |
| title_fullStr | Efficient nursery production and multiple-shoot clumps formation from shoot tiller-derived shoot apices of dwarf napiergrass (Pennisetum purpureum Schumach.) |
| title_full_unstemmed | Efficient nursery production and multiple-shoot clumps formation from shoot tiller-derived shoot apices of dwarf napiergrass (Pennisetum purpureum Schumach.) |
| title_short | Efficient nursery production and multiple-shoot clumps formation from shoot tiller-derived shoot apices of dwarf napiergrass (Pennisetum purpureum Schumach.) |
| title_sort | efficient nursery production and multiple shoot clumps formation from shoot tiller derived shoot apices of dwarf napiergrass pennisetum purpureum schumach |
| topic | Agricultural Land Management Agronomy Husbandry |
| url | https://repository.ugm.ac.id/135610/1/JOURNAL%20WARAS%202012.pdf |
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